CAS OpenIR  > 中科院上海原子核所2003年前
Modulation of the interactions of isolated ryanodine receptors of rabbit skeletal muscle by Na+ and K+
Hu, XF; Chen, KY; Xia, RH; Xu, YH; Sun, JL; Hu, J(胡钧); Zhu, PH
2003
Source PublicationBIOCHEMISTRY
ISSN0006-2960
Volume42Issue:18Pages:5515
AbstractRyanodine receptors (RyRs) of skeletal muscle, as calcium release channels, have been found to form semicrystalline arrays in the membrane of sarcoplasmic reticulum. Recently, both experimental observations and theoretical simulations suggested cooperative coupling within interlocking RyRs. To better understand the interactions between RyRs and their modulation, the aggregation and dissociation of isolated RyRs in aqueous medium containing various Na+ and K+ concentrations were investigated using photon correlation spectroscopy (PCS) and atomic force microscopy (AFM). RyRs aggregated readily at low salt concentrations. However, a different behavior was observed in the presence of Na+ or K+. Detectable aggregates were formed in 5 mug/mL RyR sample when the concentration of Na+ and K+ was reduced from 1 M to below 0.28 and 0.23 M, respectively. The dissociation of RyR aggregates was also examined when raising the salt concentration. While aggregates formed in 0.15 M NaCl medium could reverse almost completely, those formed in 0.15 M KCl medium only dissolved partly. When keeping the total salt concentration at 0.15 M, the aggregation and dissociation of RyRs were seen to evidently depend on the relative concentration of Na+ and K+. The interaction between RyRs was strengthened with increasing Na+/K+ ratios in the mixed medium. Accompanying this, a decrease of [H-3]ryanodine binding occurred. The results obtained with PCS and AFM provide further evidence for the interaction between RyRs and suggest the importance of Na+, K+, and their relative composition in modulating the interaction and cooperation between RyRs in vivo.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000182726100038
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/10061
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
Hu, XF,Chen, KY,Xia, RH,et al. Modulation of the interactions of isolated ryanodine receptors of rabbit skeletal muscle by Na+ and K+[J]. BIOCHEMISTRY,2003,42(18):5515.
APA Hu, XF.,Chen, KY.,Xia, RH.,Xu, YH.,Sun, JL.,...&Zhu, PH.(2003).Modulation of the interactions of isolated ryanodine receptors of rabbit skeletal muscle by Na+ and K+.BIOCHEMISTRY,42(18),5515.
MLA Hu, XF,et al."Modulation of the interactions of isolated ryanodine receptors of rabbit skeletal muscle by Na+ and K+".BIOCHEMISTRY 42.18(2003):5515.
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