CAS OpenIR  > 中科院上海原子核所2003年前
Real time observation of the photocleavage of single DNA molecules
Li, B(李宾); Hu, J(胡钧); Wang, Y(汪颖); Wu, SY; Huang, YB(黄一波); Li, MQ(李民乾)
2003
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume48Issue:7Pages:673
AbstractA method for real time observation of photo-cleavage of stretched lambdaDNA at single molecular level by a fluorescent microscope coupled with CCD is developed. DNA molecules stained with YOYO-1 are stretched by the molecular combing technique and fixed on a modified slide. Then the process of photocleavage and relaxation of DNA under radiation of blue light is observed. We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleavage DNA molecules. The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity, cancer research as well as the interaction between DNA and dyes.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000182411500015
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/10143
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
Li, B,Hu, J,Wang, Y,et al. Real time observation of the photocleavage of single DNA molecules[J]. CHINESE SCIENCE BULLETIN,2003,48(7):673.
APA Li, B,Hu, J,Wang, Y,Wu, SY,Huang, YB,&Li, MQ.(2003).Real time observation of the photocleavage of single DNA molecules.CHINESE SCIENCE BULLETIN,48(7),673.
MLA Li, B,et al."Real time observation of the photocleavage of single DNA molecules".CHINESE SCIENCE BULLETIN 48.7(2003):673.
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