CAS OpenIR  > 中科院上海原子核所2003年前
Stretching and imaging studies of single DNA molecules
Zhang, Y(张益); Chen, SF; Ouyang, ZQ; Hu, J(胡钧); Xiong, QH; Li, B(李宾); Huang, YB(黄一波); Li, MQ(李民乾); Jin, CZ
2000
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume45Issue:15Pages:1365
AbstractDNA molecules were stretched on silanized mica surface with the molecular combing technique, and detected with fluorescence microscopy and atomic force microscopy. Meantime, DNA molecules were stretched with a modified dynamic molecular combing technique and studied with atomic force microscopy. The results indicate that, compared with the dynamic molecular combing technique, the modified dynamic molecular combing technique has advantages of less-sample demand and less contamination to sample; as compared with the molecular combing technique, it has better aligning effect and reproducibility. Combination of this kind of DNA molecular manipulating technique with the single DNA molecule detecting technique by atomic force microscopy and fluorescence microscopy will play an important role in the basic research of molecular dynamics and the application of gene research.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000089247500006
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/10171
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
Zhang, Y,Chen, SF,Ouyang, ZQ,et al. Stretching and imaging studies of single DNA molecules[J]. CHINESE SCIENCE BULLETIN,2000,45(15):1365.
APA Zhang, Y.,Chen, SF.,Ouyang, ZQ.,Hu, J.,Xiong, QH.,...&Jin, CZ.(2000).Stretching and imaging studies of single DNA molecules.CHINESE SCIENCE BULLETIN,45(15),1365.
MLA Zhang, Y,et al."Stretching and imaging studies of single DNA molecules".CHINESE SCIENCE BULLETIN 45.15(2000):1365.
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