Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Stretching and imaging studies of single DNA molecules | |
| Zhang, Y（张益）; Chen, SF; Ouyang, ZQ; Hu, J（胡钧）; Xiong, QH; Li, B（李宾）; Huang, YB（黄一波）; Li, MQ（李民乾）; Jin, CZ | |
| 2000 | |
| Source Publication | CHINESE SCIENCE BULLETIN
 |
| ISSN | 1001-6538 |
| Volume | 45Issue:15Pages:1365 |
| Abstract | DNA molecules were stretched on silanized mica surface with the molecular combing technique, and detected with fluorescence microscopy and atomic force microscopy. Meantime, DNA molecules were stretched with a modified dynamic molecular combing technique and studied with atomic force microscopy. The results indicate that, compared with the dynamic molecular combing technique, the modified dynamic molecular combing technique has advantages of less-sample demand and less contamination to sample; as compared with the molecular combing technique, it has better aligning effect and reproducibility. Combination of this kind of DNA molecular manipulating technique with the single DNA molecule detecting technique by atomic force microscopy and fluorescence microscopy will play an important role in the basic research of molecular dynamics and the application of gene research. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000089247500006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/10171 |
| Collection | 中科院上海原子核所2003年前 |
| Recommended Citation GB/T 7714 | Zhang, Y,Chen, SF,Ouyang, ZQ,et al. Stretching and imaging studies of single DNA molecules[J]. CHINESE SCIENCE BULLETIN,2000,45(15):1365. |
| APA | Zhang, Y.,Chen, SF.,Ouyang, ZQ.,Hu, J.,Xiong, QH.,...&Jin, CZ.(2000).Stretching and imaging studies of single DNA molecules.CHINESE SCIENCE BULLETIN,45(15),1365. |
| MLA | Zhang, Y,et al."Stretching and imaging studies of single DNA molecules".CHINESE SCIENCE BULLETIN 45.15(2000):1365. |
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