Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Structural transformation and aggregation of human alpha-synuclein in trifluoroethanol: Non-amyloid component sequence is essential and beta-sheet formation is prerequisite to aggregation | |
| Li, HT; Du, HN; Tang, L（唐琳）; Hu, J（胡钧）; Hu, HY | |
| 2002 | |
| Source Publication | BIOPOLYMERS
 |
| ISSN | 0006-3525 |
| Volume | 64Issue:4Pages:221 |
| Abstract | Amyloid-like aggregation of alpha-synuclein and deposit in Lewy bodies arc thought to be the major cause of Parkinson's disease. Here we describe the secondary structural transformation and aggregation of human alpha-synuclein and its C-terminus truncated fragments in trifluoroethanol. Proteins containing the NAC (non-amyloid component) segment undergo a three-state transition: from native random coil to beta-sheet and to alpha-helical structure, while the NAC deficient fragment and gamma-synuclein undergo a typical two-state coil-to-alpha transition. The beta-sheet form is highly hydrophobic that strongly binds to 1-anilinonaphthalene-8-sulfonic acid (ANS) and is prone to self-aggregation. The results suggest that the NAC sequence is essential to beta-sheet formation and the aggregation originates from the beta-sheet intermediate, which may be implicated in the pathogenesis of Parkinson's disease. (C) 2002 Wiley Periodicals, Inc. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000176388900005 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/10173 |
| Collection | 中科院上海原子核所2003年前 |
| Recommended Citation GB/T 7714 | Li, HT,Du, HN,Tang, L,et al. Structural transformation and aggregation of human alpha-synuclein in trifluoroethanol: Non-amyloid component sequence is essential and beta-sheet formation is prerequisite to aggregation[J]. BIOPOLYMERS,2002,64(4):221. |
| APA | Li, HT,Du, HN,Tang, L,Hu, J,&Hu, HY.(2002).Structural transformation and aggregation of human alpha-synuclein in trifluoroethanol: Non-amyloid component sequence is essential and beta-sheet formation is prerequisite to aggregation.BIOPOLYMERS,64(4),221. |
| MLA | Li, HT,et al."Structural transformation and aggregation of human alpha-synuclein in trifluoroethanol: Non-amyloid component sequence is essential and beta-sheet formation is prerequisite to aggregation".BIOPOLYMERS 64.4(2002):221. |
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