CAS OpenIR  > 中科院上海原子核所2003年前
The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins
Zhang, SB; Huang, J; Zhao, H; Zhang, Y(张益); Hou, CH; Cheng, XD; Jiang, C; Li, MQ(李民乾); Hu, J(胡钧); Qian, RL
2003
Source PublicationCELL RESEARCH
ISSN1001-0602
Volume13Issue:5Pages:351
AbstractUsing atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1MNaCl. When the salt concentration was slowly reduced to 650 mM and 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic "beads-on-a-string" structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution of nucleosome, the mono- and di- nucleosomes were assembled in vitro with both HS2core (- 10681 to - 10970 bp) and NCR2 (-3 72 to - 194 bp) DNA sequences in the 5'flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be critical in the process of nucleosome assembly, recruitment of chromatin-modifying activities, and the regulation of human b-globin gene expression.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000187509700006
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/10220
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
Zhang, SB,Huang, J,Zhao, H,et al. The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins[J]. CELL RESEARCH,2003,13(5):351.
APA Zhang, SB.,Huang, J.,Zhao, H.,Zhang, Y.,Hou, CH.,...&Qian, RL.(2003).The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins.CELL RESEARCH,13(5),351.
MLA Zhang, SB,et al."The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins".CELL RESEARCH 13.5(2003):351.
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