CAS OpenIR  > 中科院上海原子核所2003年前
DNA单分子有序化测序方法
胡钧; 李民乾; 吕军鸿
Date Available2013-01-23
Country中国
Subtype发明专利
Abstract一种DNA单分子有序化测序的方法,该方法包 括下列步骤:(1)将DNA样品溶液滴加在基底表面,利用“分 子梳”技术将DNA单分子拉直并固定;(2)利用原子力显微镜 (AFM)对样品分子进行成像;(3)利用AFM的“逐线反馈纳米 操纵技术”将DNA分子依次切隔成小片段;(4)利用“单个生 物大分子的分离方法”依次对DNA小片段进行拾取;(5)利用 PCR技术分别对所分离到的DNA分子进行扩增;(6)对扩增后 的DNA样品进行测序。
Subject AreaC12q1/68
Funding Project应物所课题组
Language中文
Status20060726 授权
Application NumberCN03115428
Patent Agent王洁
Document Type专利
Identifierhttp://ir.sinap.ac.cn/handle/331007/10393
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
胡钧,李民乾,吕军鸿. DNA单分子有序化测序方法.
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