CAS OpenIR  > 中科院上海原子核所2003年前
氚离子束标记蛋白质水解产物的氨基酸分析
周美英; 王国平
1992-03-01
Source Publication核技术
Issue02
Abstract叙述了快速、灵敏、正确地分离分析氚标记蛋白质水解产物的方法。氚化核糖核酸酶A、大豆胰蛋白酶抑制剂和弹性蛋白酶用6mol/l HCl在110℃真空玻璃管中水解24 h后进行氨基酸分析;通过梯度洗脱分离出各氨基酸残基;并使之与OPA试剂反应成衍生物。经荧光检测获得每个氨基酸残基的化学量;并用液闪法测量其放射性。实验证明在氚化蛋白质中;所有的氨基酸残基均被氚化;但各个氨基酸残基上的氚分布情况随氨基酸的结构与蛋白质的结构环境影响而不同。
Indexed ByCNKI
Language中文
Funding Project原子核所项目组
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/11197
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
周美英,王国平. 氚离子束标记蛋白质水解产物的氨基酸分析[J]. 核技术,1992(02).
APA 周美英,&王国平.(1992).氚离子束标记蛋白质水解产物的氨基酸分析.核技术(02).
MLA 周美英,et al."氚离子束标记蛋白质水解产物的氨基酸分析".核技术 .02(1992).
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