CAS OpenIR  > 中科院上海原子核所2003年前
氚离子束标记新技术
张年宝; 盛树刚; 要福增; 谢炳华; 周美英; 曹蓉珍; 俞斌伟; 钟德松; 秦锐芳; 王国平
1990-10-28
Source Publication核技术
Issue10
Abstract本文介绍一种先进的氚离子束标记技术;用于蛋白质、肽和非挥发性有机化合物的氚标记。在该技术中;加速受激发的氚离子束;去轰击固体样品;发生氢-氚交换反应。氚化大豆胰蛋白酶抑制剂、核糖核酸酶A、弹性蛋白和茯苓聚糖等十一种化合物已用此法输备。氚化粗产物经透析、离子交换色层和凝胶过滤等方法纯化后;蛋白质和聚糖的比活性一般可达37GBq/mmol以上;没有放射性副产物和具有与天然物质相同的生物活性。氚化蛋白质的氨基酸分析表明;在蛋白质中;His、Tyr和Phe残基具有较高的比活性而Val、Ile和Ser残基的比活性较低。
Indexed ByCNKI
Language中文
Funding Project原子核所项目组
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/11198
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
张年宝,盛树刚,要福增,等. 氚离子束标记新技术[J]. 核技术,1990(10).
APA 张年宝.,盛树刚.,要福增.,谢炳华.,周美英.,...&王国平.(1990).氚离子束标记新技术.核技术(10).
MLA 张年宝,et al."氚离子束标记新技术".核技术 .10(1990).
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