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高效液相色谱法测定[1,2,6,7-~3H]睾丸酮的放化纯度及比活度
周瑞菊; 潘光明; 赵夏令
1988-01-31
Source Publication核技术
Issue01
Abstract<正> 高比活度、高纯度的氚标记睾丸酮经常应用于临床医学中的放射免疫测定;其纯度与比活度直接影响着放射免疫测定的结果。我们用日立638-50型HPLC结合自制流动式软β放射性连续测量装置及双笔记录仪;测定了10μl[1;2;6;7-~3H]睾丸酮样品;可同时得到其放射性色谱峰和紫外色谱峰;根据标准曲线可求得放化活度和化学量;立即得到[1;2;6;7-~3H]睾丸酮的比活度。本法具有取样量少、步骤简单、操作安全、能定量分离分析、数据准确度高、快速灵敏等优点。
Indexed ByCNKI
Language中文
Funding Project原子核所项目组
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/11440
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
周瑞菊,潘光明,赵夏令. 高效液相色谱法测定[1,2,6,7-~3H]睾丸酮的放化纯度及比活度[J]. 核技术,1988(01).
APA 周瑞菊,潘光明,&赵夏令.(1988).高效液相色谱法测定[1,2,6,7-~3H]睾丸酮的放化纯度及比活度.核技术(01).
MLA 周瑞菊,et al."高效液相色谱法测定[1,2,6,7-~3H]睾丸酮的放化纯度及比活度".核技术 .01(1988).
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