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抗人肺腺癌单克隆抗体LC-1 IgM片段的分离、鉴定和初步应用
林斯骏; 李明; 沈瑾; 曹蓉珍; 马寄晓; 张素胤; 王珏; 葛锡锐
Source Publication中华核医学杂志
Abstract将抗人肺癌单抗LC-1经0.25.0.5、1、2、4mmol/L不同浓度的二硫苏糖醇(DTT);在37℃作用15~120分钟;得出最佳条件是将纯化LC-1IgM与1mmol/LDTT溶液混匀;37℃保温60分钟;LC-1IgM降解为不同片段;经SephadexG150柱获得纯化的片段;该片段分子量经十二烷基硫酸钠-聚丙烯酸胺凝胶电泳鉴定为118000;用ELISA法检测具有免疫活性;用 ̄(99m)Tc标记后注入带瘤裸鼠体内;能浓集于肿瘤病灶;显示该片段具有与人肺腺癌抗原特异结合的能力;有临床定位诊断的潜力.
Indexed ByCNKI
Funding Project原子核所项目组
Document Type期刊论文
Recommended Citation
GB/T 7714
林斯骏,李明,沈瑾,等. 抗人肺腺癌单克隆抗体LC-1 IgM片段的分离、鉴定和初步应用[J]. 中华核医学杂志,1994(04).
APA 林斯骏.,李明.,沈瑾.,曹蓉珍.,马寄晓.,...&葛锡锐.(1994).抗人肺腺癌单克隆抗体LC-1 IgM片段的分离、鉴定和初步应用.中华核医学杂志(04).
MLA 林斯骏,et al."抗人肺腺癌单克隆抗体LC-1 IgM片段的分离、鉴定和初步应用".中华核医学杂志 .04(1994).
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