Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol | |
| Liu, G; Chen, HD; Peng, HZ（彭红珍）; Song, SP（宋世平）; Gao, JM; Lu, JX; Ding, M; Li, LY; Ren, SZ; Zou, ZY; Fan, CH（樊春海） | |
| 2011 | |
| Source Publication | BIOSENSORS & BIOELECTRONICS
 |
| ISSN | 0956-5663 |
| Volume | 28Issue:1Pages:308 |
| Abstract | Carbon nanotubes have shown their unique advantages of mechanical, chemical and electronic properties in bioanalysis. We herein report a new method to efficiently and reproducibly prepare multi-walled carbon nanotubes (MWNTs)-protein sensing layers for electrochemical immunosensors. This method employs centrifugation to prepare a conjugate of MWNTs and goat anti mouse-immunoglobulin G (IgG) (secondary antibody). The conjugates were then deposited on screen-printed electrodes to form a nanostructured layer (MWNT-I layer). CLB monoclonal antibody was assembled through its binding to the secondary antibody. The MWNT-I layer-based electrodes were used for rapid and sensitive amperometric immunosensing detection of clenbuterol (CLB) in swine urine samples. Horseradish peroxidase-coupled CLB (CLB-HRP) competed with free CLB in the samples to bind the monoclonal antibody. It has shown significantly higher sensitivity and better reproducibility than the chemical conjugation method. This MWNT-based immunosensor is highly sensitive, leading to a limit of detection of 0.1 ng/mL within a rapid assay time of 16 min. Its sensitivity is at least 1 order of magnitude higher than that of a normal immunosensor (without MWNTs). The sensing device is portable with disposable screen-printed electrode, satisfactorily meeting the requirements for field detection of food security-related species. (C) 2011 Elsevier B.V. All rights reserved. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000295661700047 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/12843 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Liu, G,Chen, HD,Peng, HZ,et al. A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol[J]. BIOSENSORS & BIOELECTRONICS,2011,28(1):308. |
| APA | Liu, G.,Chen, HD.,Peng, HZ.,Song, SP.,Gao, JM.,...&Fan, CH.(2011).A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol.BIOSENSORS & BIOELECTRONICS,28(1),308. |
| MLA | Liu, G,et al."A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol".BIOSENSORS & BIOELECTRONICS 28.1(2011):308. |
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