CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol
Liu, G; Chen, HD; Peng, HZ(彭红珍); Song, SP(宋世平); Gao, JM; Lu, JX; Ding, M; Li, LY; Ren, SZ; Zou, ZY; Fan, CH(樊春海)
2011
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume28Issue:1Pages:308
AbstractCarbon nanotubes have shown their unique advantages of mechanical, chemical and electronic properties in bioanalysis. We herein report a new method to efficiently and reproducibly prepare multi-walled carbon nanotubes (MWNTs)-protein sensing layers for electrochemical immunosensors. This method employs centrifugation to prepare a conjugate of MWNTs and goat anti mouse-immunoglobulin G (IgG) (secondary antibody). The conjugates were then deposited on screen-printed electrodes to form a nanostructured layer (MWNT-I layer). CLB monoclonal antibody was assembled through its binding to the secondary antibody. The MWNT-I layer-based electrodes were used for rapid and sensitive amperometric immunosensing detection of clenbuterol (CLB) in swine urine samples. Horseradish peroxidase-coupled CLB (CLB-HRP) competed with free CLB in the samples to bind the monoclonal antibody. It has shown significantly higher sensitivity and better reproducibility than the chemical conjugation method. This MWNT-based immunosensor is highly sensitive, leading to a limit of detection of 0.1 ng/mL within a rapid assay time of 16 min. Its sensitivity is at least 1 order of magnitude higher than that of a normal immunosensor (without MWNTs). The sensing device is portable with disposable screen-printed electrode, satisfactorily meeting the requirements for field detection of food security-related species. (C) 2011 Elsevier B.V. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000295661700047
Citation statistics
Cited Times:82[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/12843
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Liu, G,Chen, HD,Peng, HZ,et al. A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol[J]. BIOSENSORS & BIOELECTRONICS,2011,28(1):308.
APA Liu, G.,Chen, HD.,Peng, HZ.,Song, SP.,Gao, JM.,...&Fan, CH.(2011).A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol.BIOSENSORS & BIOELECTRONICS,28(1),308.
MLA Liu, G,et al."A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol".BIOSENSORS & BIOELECTRONICS 28.1(2011):308.
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