CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I
Xu, H; Gao, SL; Liu, QW; Pan, D(潘敦); Wang, LH(王丽华); Ren, SZ; Ding, M; Chen, JW; Liu, G
2011
Source PublicationSENSORS
ISSN1424-8220
Volume11Issue:11Pages:10187
AbstractWe here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000297698600013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/12846
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Xu, H,Gao, SL,Liu, QW,et al. A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I[J]. SENSORS,2011,11(11):10187.
APA Xu, H.,Gao, SL.,Liu, QW.,Pan, D.,Wang, LH.,...&Liu, G.(2011).A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I.SENSORS,11(11),10187.
MLA Xu, H,et al."A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I".SENSORS 11.11(2011):10187.
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