Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface | |
| Liu, G; Lao, RJ; Xu, L; Xu, Q; Li, LY; Zhang, M; Shen, H; Mathur, S; Fan, CH（樊春海）; Song, SP（宋世平） | |
| 2011 | |
| Source Publication | SENSORS
 |
| ISSN | 1424-8220 |
| Volume | 11Issue:8Pages:8018 |
| Abstract | We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000294253900042 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/12853 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Recommended Citation GB/T 7714 | Liu, G,Lao, RJ,Xu, L,et al. Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface[J]. SENSORS,2011,11(8):8018. |
| APA | Liu, G.,Lao, RJ.,Xu, L.,Xu, Q.,Li, LY.,...&Song, SP.(2011).Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface.SENSORS,11(8),8018. |
| MLA | Liu, G,et al."Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface".SENSORS 11.8(2011):8018. |
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