CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface
Liu, G; Lao, RJ; Xu, L; Xu, Q; Li, LY; Zhang, M; Shen, H; Mathur, S; Fan, CH(樊春海); Song, SP(宋世平)
2011
Source PublicationSENSORS
ISSN1424-8220
Volume11Issue:8Pages:8018
AbstractWe report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000294253900042
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/12853
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Liu, G,Lao, RJ,Xu, L,et al. Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface[J]. SENSORS,2011,11(8):8018.
APA Liu, G.,Lao, RJ.,Xu, L.,Xu, Q.,Li, LY.,...&Song, SP.(2011).Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface.SENSORS,11(8),8018.
MLA Liu, G,et al."Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface".SENSORS 11.8(2011):8018.
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