CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein
Zhang, QZ; Zhao, B; Yan, J(颜娟); Song, SP(宋世平); Min, R; Fan, CH(樊春海)
2011
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume83Issue:23Pages:9191
AbstractWe have developed a nanotube-based colorimetric probe using multiwalled carbon nanotubes (MWNTs), anti-immunoglobulin G (anti-IgG), and horseradish peroxidase (HRP). The probe was used as an alternative to conventional colorimetric conjugates to obtain amplified signals in a sandwich-type immunoassay for ataxia telangiectasia mutated (ATM), a potential biomarker for radiation doses and cancers. Results show that the MWNT-based probe colorimetry was 5000 times more sensitive than a conventional ELISA, while its concentration range was 10 000 times wider than that of the latter. Its limit of detection (LOD) was 0.2, fg/mL (54 aM, similar to 32 molecules in 1 mu L samples). Control experiments showed that detection of ATM molecules at the picogram-level could still be achieved in samples that contained protein makers present at more than 100 times the ATM concentration, demonstrating the high specificity of the technique. The MWNT-based probe also has the potential to become a universal probe for colorimetric assays of most protein markers because it can recognize the associated rabbit polyclonal antibodies.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000297481700054
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/12871
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zhang, QZ,Zhao, B,Yan, J,et al. Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein[J]. ANALYTICAL CHEMISTRY,2011,83(23):9191.
APA Zhang, QZ,Zhao, B,Yan, J,Song, SP,Min, R,&Fan, CH.(2011).Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein.ANALYTICAL CHEMISTRY,83(23),9191.
MLA Zhang, QZ,et al."Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein".ANALYTICAL CHEMISTRY 83.23(2011):9191.
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