CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Self-Assembled Multivalent DNA Nanostructures for Noninvasive Intracellular Delivery of Immunostimulatory CpG Oligonucleotides
Li, J; Pei, H; Zhu, B; Liang, L; Wei, M; He, Y; Chen, N; Li, D(李迪); Huang, Q(黄庆); Fan, CH(樊春海)
2011
Source PublicationACS NANO
ISSN1936-0851
Volume5Issue:11Pages:8783
AbstractDesigned oligonucleotides-can self-assemble Into DNA nanostructures with well-defined structures and uniform sizes, which provide unprecedented opportunities for biosensing, molecular imaging, and drug delivery. In this work, we have developed functional, multivalent DNA nanostructures by appending unmethylated CpG motifs to three-dimensional DNA tetrahedra. These small-sized functional nanostructures are compact, mechanically stable, and noncytotoxic. We have demonstrated that DNA nanostructures are resistant to nuclease degradation and remain substantially intact in fetal bovine serum and in cells for at least several hours. Significantly, these functional nanostructures can noninvasively and efficiently enter macrophage-like RAW264.7 cells without the aid of transfection agents. After they are uptaken by cells, CpG motifs are recognized by the Toll-like receptor 9 (TLR9) that activates downstream pathways to induce immunostimulatory effects, producing high-level secretion of various pro-inflammatory cytokines including tumor necrosis factor (INF)-alpha., interleukin (IL)-6, and IL-12. We also show that multivalent CpG motifs greatly enhance the immunostimulatory effect of the nanostructures. Given the high efficacy of these functional nanostructures and their noncytotoxic nature, we expect that DNA nanostructures will become a promising tool for targeted drug delivery.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000297143300037
Citation statistics
Cited Times:396[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/12878
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, J,Pei, H,Zhu, B,et al. Self-Assembled Multivalent DNA Nanostructures for Noninvasive Intracellular Delivery of Immunostimulatory CpG Oligonucleotides[J]. ACS NANO,2011,5(11):8783.
APA Li, J.,Pei, H.,Zhu, B.,Liang, L.,Wei, M.,...&Fan, CH.(2011).Self-Assembled Multivalent DNA Nanostructures for Noninvasive Intracellular Delivery of Immunostimulatory CpG Oligonucleotides.ACS NANO,5(11),8783.
MLA Li, J,et al."Self-Assembled Multivalent DNA Nanostructures for Noninvasive Intracellular Delivery of Immunostimulatory CpG Oligonucleotides".ACS NANO 5.11(2011):8783.
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