CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel
Zhang, Xu; Ren, Wenlin; DeCaen, Paul; Yan, Chuangye; Tao, Xiao; Tang, Lin(唐琳); Wang, Jingjing; Hasegawa, Kazuya; Kumasaka, Takashi; He, Jianhua(何建华); Wang, Jiawei; Clapham, David E.; Yan, Nieng
2012
Source PublicationNATURE
ISSN0028-0836
Volume486Issue:7401Pages:130
AbstractVoltage-gated sodium (Na-v) channels are essential for the rapid depolarization of nerve and muscle(1), and are important drug targets(2). Determination of the structures of Na-v channels will shed light on ion channel mechanisms and facilitate potential clinical applications. A family of bacterial Na-v channels, exemplified by the Na+-selective channel of bacteria (NaChBac)(3), provides a useful model system for structure-function analysis. Here we report the crystal structure of NavRh, a NaChBac orthologue from the marine alphaproteobacterium HIMB114 (Rickettsiales sp. HIMB114; denoted Rh), at 3.05 angstrom resolution. The channel comprises an asymmetric tetramer. The carbonyl oxygen atoms of Thr 178 and Leu 179 constitute an inner site within the selectivity filter where a hydrated Ca2+ resides in the crystal structure. The outer mouth of the Na+ selectivity filter, defined by Ser 181 and Glu 183, is closed, as is the activation gate at the intracellular side of the pore. The voltage sensors adopt a depolarized conformation in which all the gating charges are exposed to the extracellular environment. We propose that NavRh is in an 'inactivated' conformation. Comparison of NavRh with Na(v)Ab(4) reveals considerable conformational rearrangements that may underlie the electromechanical coupling mechanism of voltage-gated channels.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000304854000042
Citation statistics
Cited Times:333[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/12895
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Zhang, Xu,Ren, Wenlin,DeCaen, Paul,et al. Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel[J]. NATURE,2012,486(7401):130.
APA Zhang, Xu.,Ren, Wenlin.,DeCaen, Paul.,Yan, Chuangye.,Tao, Xiao.,...&Yan, Nieng.(2012).Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.NATURE,486(7401),130.
MLA Zhang, Xu,et al."Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel".NATURE 486.7401(2012):130.
Files in This Item: Download All
File Name/Size DocType Version Access License
Crystal structure of(1954KB) 开放获取LicenseView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Xu]'s Articles
[Ren, Wenlin]'s Articles
[DeCaen, Paul]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Xu]'s Articles
[Ren, Wenlin]'s Articles
[DeCaen, Paul]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Xu]'s Articles
[Ren, Wenlin]'s Articles
[DeCaen, Paul]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.