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Highly Sensitive Electrochemical Sensor for Mercury(II) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based Amplification
Zhu, ZQ; Su, YY; Li, J; Li, D(李迪); Zhang, J; Song, SP(宋世平); Zhao, Y; Li, GX; Fan, CH(樊春海)
2009
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume81Issue:18Pages:7660
AbstractWe report a highly sensitive electrochemical sensor for the detection of Hg(2+) ions in aqueous solution by using a thymine (T)-rich, mercury-specific oligonucleotide (MSO) probe and gold nanoparticles (Au NPs)-based signal amplification. The MSO probe contains seven thymine bases at both ends and a "mute" spacer in the middle, which, in the presence of Hg(2+), forms a hairpin structure via the Hg(2+)-mediated coordination of T-Hg(2+)-T base pairs. The thiolated MSO probe is immobilized on Au electrodes to capture free Hg(2+) in aqueous media, and the MSO-bound Hg(2+) can be electrochemically reduced to Hg(+), which provides a readout signal for quantitative detection of Hg(2+). This direct immobilization strategy leads to a detection limit of 1 mu M. In order to improve the sensitivity, MSO probe-modified Au NPs are employed to amplify the electrochemical signals. Au NPs are comodified with the MSO probe and a linking probe that is complementary to a capture DNA probe immobilized on gold electrodes. We demonstrated that this Au NPs-based sensing strategy brings about an amplification factor of more than 3 orders of magnitude, leading to a limit of detection of 0.5 nM (100 ppt), which satisfactorily meets the sensitivity requirement of U.S. Environmental Protection Agency (EPA). This Au NPs-based Hg(2+) sensor also exhibits excellent selectivity over a spectrum of interference metal ions. Considering the high sensitivity and selectivity of this sensor, as well as the cost-effective and portable features of electrochemical techniques, we expect this Au NPs amplified electrochemical sensor will be a promising candidate for field detection of environmentally toxic mercury.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000269656700019
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13017
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Zhu, ZQ,Su, YY,Li, J,et al. Highly Sensitive Electrochemical Sensor for Mercury(II) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based Amplification[J]. ANALYTICAL CHEMISTRY,2009,81(18):7660.
APA Zhu, ZQ.,Su, YY.,Li, J.,Li, D.,Zhang, J.,...&Fan, CH.(2009).Highly Sensitive Electrochemical Sensor for Mercury(II) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based Amplification.ANALYTICAL CHEMISTRY,81(18),7660.
MLA Zhu, ZQ,et al."Highly Sensitive Electrochemical Sensor for Mercury(II) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based Amplification".ANALYTICAL CHEMISTRY 81.18(2009):7660.
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