CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Modulation of DNA Polymerases with Gold Nanoparticles and their Applications in Hot-Start PCR
Mi, LJ(米丽娟); Wen, YQ; Pan, D; Wang, YH; Fan, CH(樊春海); Hu, J(胡钧)
2009
Source PublicationSMALL
ISSN1613-6810
Volume5Issue:22Pages:2597
AbstractA new gold-nanoparticle (AuNP)-based strategy to dynamically modulate the activity of DNA polymerases and realize a hot-start (HS)-like effect in the polymerase chain reaction (PCR) is reported, which effectively prevents unwanted nonspecific amplification and improves the performance of PCRs. A high-fidelity Pfu DNA polymerase is employed as the model system. Interestingly, AuNPs inactivate the polymerase activity of Pfu at low temperature, thus resembling an. antibody-based HS PCR. This inhibition effect of AuNPs is demonstrated for the preamplification, polymerization activity of the PCR, which largely suppresses nonspecific amplification at temperatures between 30 and 60 degrees C and leads to highly specific and sensitive PCR amplification with Pfu. Significantly, the fidelity of Pfu is not sacrificed in the presence of AuNPs. Therefore, this AuNP-based HS strategy provides a straightforward and potentially versatile approach to realize high-performance PCR amplification.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000272224400017
Citation statistics
Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13019
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Mi, LJ,Wen, YQ,Pan, D,et al. Modulation of DNA Polymerases with Gold Nanoparticles and their Applications in Hot-Start PCR[J]. SMALL,2009,5(22):2597.
APA Mi, LJ,Wen, YQ,Pan, D,Wang, YH,Fan, CH,&Hu, J.(2009).Modulation of DNA Polymerases with Gold Nanoparticles and their Applications in Hot-Start PCR.SMALL,5(22),2597.
MLA Mi, LJ,et al."Modulation of DNA Polymerases with Gold Nanoparticles and their Applications in Hot-Start PCR".SMALL 5.22(2009):2597.
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