CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection
Zhou, YT; Huang, Q(黄庆); Gao, JM; Lu, JX; Shen, XZ; Fan, CH(樊春海)
2010
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume38Issue:15
AbstractWe herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000281345900005
Citation statistics
Cited Times:143[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13021
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Zhou, YT,Huang, Q,Gao, JM,et al. A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection[J]. NUCLEIC ACIDS RESEARCH,2010,38(15).
APA Zhou, YT,Huang, Q,Gao, JM,Lu, JX,Shen, XZ,&Fan, CH.(2010).A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection.NUCLEIC ACIDS RESEARCH,38(15).
MLA Zhou, YT,et al."A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection".NUCLEIC ACIDS RESEARCH 38.15(2010).
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