Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Amplified Fluorescent Recognition of G-Quadruplex Folding with a Cationic Conjugated Polymer and DNA Intercalator | |
| Xu, H; Gao, SL; Yang, Q; Pan, D; Wang, LH(王丽华）; Fan, CH（樊春海） | |
| 2010 | |
| Source Publication | ACS APPLIED MATERIALS & INTERFACES
 |
| ISSN | 1944-8244 |
| Volume | 2Issue:11Pages:3211 |
| Abstract | The single stranded DNA (ssDNA) with G-rich sequence can fold into G-quadruplex vial intramolecular hydrogen bonding interaction in the presence of ligand. This structure conversion can be specifically detected by a fluorescence method based on different interaction between SYBR Green I (SG) and various DNA structures. SG is proved to intercalate into G-quadruplex and results in high fluorescence intensity which can be further amplified by 6-fold through fluorescence resonance energy transfer (FRET) from a water-soluble cationic conjugated polymer (CCP) to SG due to the high affinity of positively charged CCP to negatively charged rigid G-quadruplex whereas it is not performed for ssDNA in the absence of K. As a result, the ssDNA/SG/CCP complex can be used to detect potassium ions with improved selectivity in a label-free and cost-effective manner. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000284454400035 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13027 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Recommended Citation GB/T 7714 | Xu, H,Gao, SL,Yang, Q,et al. Amplified Fluorescent Recognition of G-Quadruplex Folding with a Cationic Conjugated Polymer and DNA Intercalator[J]. ACS APPLIED MATERIALS & INTERFACES,2010,2(11):3211. |
| APA | Xu, H,Gao, SL,Yang, Q,Pan, D,Wang, LH,&Fan, CH.(2010).Amplified Fluorescent Recognition of G-Quadruplex Folding with a Cationic Conjugated Polymer and DNA Intercalator.ACS APPLIED MATERIALS & INTERFACES,2(11),3211. |
| MLA | Xu, H,et al."Amplified Fluorescent Recognition of G-Quadruplex Folding with a Cationic Conjugated Polymer and DNA Intercalator".ACS APPLIED MATERIALS & INTERFACES 2.11(2010):3211. |
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