Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A | |
| Pu, KY; Shi, JB; Wang, LH（王丽华）; Cai, LP; Wang, GA; Liu, B | |
| 2010 | |
| Source Publication | MACROMOLECULES
 |
| ISSN | 0024-9297 |
| Volume | 43Issue:23Pages:9690 |
| Abstract | Mannose-substituted blue-fluorescent cationic conjugated polyelectrolyte (131) and yellow-fluorescent neutral conjugated oligomer (6) are synthesized via the combination of Suzuki coupling polymerization and click chemistry. The spectral overlap between the emission of P1 and the absorption of 6 allows one to blend them to form an intelligent fluorescence resonance energy transfer (FRET) pair for multicolor protein sensing. Despite the nonspecific-interaction-induced perturbation in the polymer emission, strong specific binding between mannose and Concanavalin A (ConA) is able to result in protein-selective FRET from PI to 6. The fluorescence of 6/P1 blend in the presence of ConA is dominated by yellow emission, while it remains blue in the presence of other six nonspecific proteins. The 6/P1 blend thus serves as a multicolor bicomponent FRET probe for label-free visual detection of ConA in a high-contrast and convenient manner. In addition, its linear ratiometric fluorescence response toward ConA enables effective protein quantification with a low limit of detection of similar to 1.5 nM. This study demonstrates the importance of molecular engineering in conjugated polyelectrolyte based label-free chemical and biological sensing. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000284967000018 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13045 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Recommended Citation GB/T 7714 | Pu, KY,Shi, JB,Wang, LH,et al. Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A[J]. MACROMOLECULES,2010,43(23):9690. |
| APA | Pu, KY,Shi, JB,Wang, LH,Cai, LP,Wang, GA,&Liu, B.(2010).Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A.MACROMOLECULES,43(23),9690. |
| MLA | Pu, KY,et al."Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A".MACROMOLECULES 43.23(2010):9690. |
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