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Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A
Pu, KY; Shi, JB; Wang, LH(王丽华); Cai, LP; Wang, GA; Liu, B
2010
Source PublicationMACROMOLECULES
ISSN0024-9297
Volume43Issue:23Pages:9690
AbstractMannose-substituted blue-fluorescent cationic conjugated polyelectrolyte (131) and yellow-fluorescent neutral conjugated oligomer (6) are synthesized via the combination of Suzuki coupling polymerization and click chemistry. The spectral overlap between the emission of P1 and the absorption of 6 allows one to blend them to form an intelligent fluorescence resonance energy transfer (FRET) pair for multicolor protein sensing. Despite the nonspecific-interaction-induced perturbation in the polymer emission, strong specific binding between mannose and Concanavalin A (ConA) is able to result in protein-selective FRET from PI to 6. The fluorescence of 6/P1 blend in the presence of ConA is dominated by yellow emission, while it remains blue in the presence of other six nonspecific proteins. The 6/P1 blend thus serves as a multicolor bicomponent FRET probe for label-free visual detection of ConA in a high-contrast and convenient manner. In addition, its linear ratiometric fluorescence response toward ConA enables effective protein quantification with a low limit of detection of similar to 1.5 nM. This study demonstrates the importance of molecular engineering in conjugated polyelectrolyte based label-free chemical and biological sensing.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000284967000018
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13045
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Pu, KY,Shi, JB,Wang, LH,et al. Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A[J]. MACROMOLECULES,2010,43(23):9690.
APA Pu, KY,Shi, JB,Wang, LH,Cai, LP,Wang, GA,&Liu, B.(2010).Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A.MACROMOLECULES,43(23),9690.
MLA Pu, KY,et al."Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A".MACROMOLECULES 43.23(2010):9690.
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