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Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces
Wan, Y; Lao, RJ; Liu, G; Song, SP(宋世平); Wang, LH(王丽华); Li, D(李迪); Fan, CH(樊春海)
2010
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume114Issue:19Pages:6703
AbstractIn this work, we report a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection by using oligonucleotide-incorporated nonfouling surfaces (ONS). A typical "sandwich" scheme was employed to perform the SNP assay. The presence of the target DNA templated the ligation between the capture probe DNA anchored on gold electrodes and the tandem reporter probe tagged with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. This method could effectively differentiate SNP sites with only one-base mismatch. Importantly, the differentiation ratio was significantly higher than that with surfaces without the nonfouling property, which clearly demonstrated the superiority of the ONS strategy.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000277499700054
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13046
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Wan, Y,Lao, RJ,Liu, G,et al. Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2010,114(19):6703.
APA Wan, Y.,Lao, RJ.,Liu, G.,Song, SP.,Wang, LH.,...&Fan, CH.(2010).Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces.JOURNAL OF PHYSICAL CHEMISTRY B,114(19),6703.
MLA Wan, Y,et al."Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces".JOURNAL OF PHYSICAL CHEMISTRY B 114.19(2010):6703.
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