Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces | |
| Wan, Y; Lao, RJ; Liu, G; Song, SP（宋世平）; Wang, LH（王丽华）; Li, D（李迪）; Fan, CH（樊春海） | |
| 2010 | |
| Source Publication | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| ISSN | 1520-6106 |
| Volume | 114Issue:19Pages:6703 |
| Abstract | In this work, we report a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection by using oligonucleotide-incorporated nonfouling surfaces (ONS). A typical "sandwich" scheme was employed to perform the SNP assay. The presence of the target DNA templated the ligation between the capture probe DNA anchored on gold electrodes and the tandem reporter probe tagged with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. This method could effectively differentiate SNP sites with only one-base mismatch. Importantly, the differentiation ratio was significantly higher than that with surfaces without the nonfouling property, which clearly demonstrated the superiority of the ONS strategy. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000277499700054 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13046 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Recommended Citation GB/T 7714 | Wan, Y,Lao, RJ,Liu, G,et al. Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2010,114(19):6703. |
| APA | Wan, Y.,Lao, RJ.,Liu, G.,Song, SP.,Wang, LH.,...&Fan, CH.(2010).Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces.JOURNAL OF PHYSICAL CHEMISTRY B,114(19),6703. |
| MLA | Wan, Y,et al."Multiplexed Electrochemical DNA Sensor for Single-Nucleotide Polymorphism Typing by Using Oligonucleotide-Incorporated Nonfouling Surfaces".JOURNAL OF PHYSICAL CHEMISTRY B 114.19(2010):6703. |
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