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Resveratrol differentially modulates inflammatory responses of microglia and astrocytes
Lu, Xiaofeng; Ma, Lili; Ruan, Lingfei; Kong, Yan; Mou, Haiwei; Zhang, Zhijie; Wang, Zhijun(王志军); Wang, Ji Ming; Le, Yingying
2010
Source PublicationJOURNAL OF NEUROINFLAMMATION
ISSN1742-2094
Volume7
AbstractBackground: Inflammatory responses in the CNS mediated by activated glial cells play an important role in host-defense but are also involved in the development of neurodegenerative diseases. Resveratrol is a natural polyphenolic compound that has cardioprotective, anticancer and anti-inflammatory properties. We investigated the capacity of resveratrol to protect microglia and astrocyte from inflammatory insults and explored mechanisms underlying different inhibitory effects of resveratrol on microglia and astrocytes. Methods: A murine microglia cell line (N9), primary microglia, or astrocytes were stimulated by LPS with or without different concentrations of resveratrol. The expression and release of proinflammatory cytokines (TNF-alpha, IL-1 beta, IL-6, MCP-1) and iNOS/NO by the cells were measured by PCR/real-time PCR and ELISA, respectively. The phosphorylation of the MAP kinase superfamily was analyzed by western blotting, and activation of NF-kappa B and AP-1 was measured by luciferase reporter assay and/or electrophoretic mobility shift assay. Results: We found that LPS stimulated the expression of TNF-alpha, IL-1 beta, IL-6, MCP-1 and iNOS in murine microglia and astrocytes in which MAP kinases, NF-kappa B and AP-1 were differentially involved. Resveratrol inhibited LPS-induced expression and release of TNF-alpha, IL-6, MCP-1, and iNOS/NO in both cell types with more potency in microglia, and inhibited LPS-induced expression of IL-1 beta in microglia but not astrocytes. Resveratrol had no effect on LPS-stimulated phosphorylation of ERK1/2 and p38 in microglia and astrocytes, but slightly inhibited LPS-stimulated phosphorylation of JNK in astrocytes. Resveratrol inhibited LPS-induced NF-kappa B activation in both cell types, but inhibited AP-1 activation only in microglia. Conclusion: These results suggest that murine microglia and astrocytes produce proinflammatory cytokines and NO in response to LPS in a similar pattern with some differences in signaling molecules involved, and further suggest that resveratrol exerts anti-inflammatory effects in microglia and astrocytes by inhibiting different proinflammatory cytokines and key signaling molecules.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000282385200001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13052
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Lu, Xiaofeng,Ma, Lili,Ruan, Lingfei,et al. Resveratrol differentially modulates inflammatory responses of microglia and astrocytes[J]. JOURNAL OF NEUROINFLAMMATION,2010,7.
APA Lu, Xiaofeng.,Ma, Lili.,Ruan, Lingfei.,Kong, Yan.,Mou, Haiwei.,...&Le, Yingying.(2010).Resveratrol differentially modulates inflammatory responses of microglia and astrocytes.JOURNAL OF NEUROINFLAMMATION,7.
MLA Lu, Xiaofeng,et al."Resveratrol differentially modulates inflammatory responses of microglia and astrocytes".JOURNAL OF NEUROINFLAMMATION 7(2010).
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