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Saturated Forster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging
Deng, SH; Chen, JF; Huang, Q(黄庆); Fan, CH(樊春海); Cheng, Y
2010
Source PublicationOPTICS LETTERS
ISSN0146-9592
Volume35Issue:23Pages:3862
AbstractWe theoretically demonstrate that the spatial resolution of stimulated emission depletion (STED) microscopy can be substantially enhanced without increasing the intensity of the STED beam. In our scheme, tiny nanobeads codoped with donor and acceptor molecules are used as fluorescent probes, in which Forster resonance energy transfer (FRET) can occur with an similar to 100% efficiency between the donors and acceptors. Enhancement of the depletion of acceptors in the nanobeads with the doughnut-shaped depletion beam can lead to an increase of FRET efficiency in the outer area of the excitation spot, which itself is used for deexciting donor molecules and, consequently, enhancing the optical resolution. (C) 2010 Optical Society of America
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000284832200002
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13053
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Deng, SH,Chen, JF,Huang, Q,et al. Saturated Forster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging[J]. OPTICS LETTERS,2010,35(23):3862.
APA Deng, SH,Chen, JF,Huang, Q,Fan, CH,&Cheng, Y.(2010).Saturated Forster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging.OPTICS LETTERS,35(23),3862.
MLA Deng, SH,et al."Saturated Forster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging".OPTICS LETTERS 35.23(2010):3862.
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