Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| The sandwich method for protein crystallization and its effect on crystal growth | |
| Sun, Lihua（孙丽华）; Li, Jian（李健）; Xu, Chunyan（徐春艳）; Yu, Feng（郁峰）; Zhou, Huan; Tang, Lin（唐琳）; He, Jianhua（何建华） | |
| 2010 | |
| Source Publication | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| ISSN | 1672-9145 |
| Volume | 42Issue:5Pages:332 |
| Abstract | A device has been invented for protein crystallization by sandwiching the liquid droplet between two surfaces, in which both hydrophilic and hydrophobic surfaces can be used as crystallization substrates. Comparing with the traditional hanging drop method, it can also reduce the evaporation rate of the liquid droplet and provide a stable environment for the crystal growth. In this work, crystal growth experiments for several proteins, especially on the hydrophilic substrate of mica, have shown the positive effect on crystal growth for improving crystallization conditions and the quality of crystals. The features of this new sandwich method and its mechanism have also been discussed. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000278421900005 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13061 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Recommended Citation GB/T 7714 | Sun, Lihua,Li, Jian,Xu, Chunyan,et al. The sandwich method for protein crystallization and its effect on crystal growth[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(5):332. |
| APA | Sun, Lihua.,Li, Jian.,Xu, Chunyan.,Yu, Feng.,Zhou, Huan.,...&He, Jianhua.(2010).The sandwich method for protein crystallization and its effect on crystal growth.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(5),332. |
| MLA | Sun, Lihua,et al."The sandwich method for protein crystallization and its effect on crystal growth".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.5(2010):332. |
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