CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Ultrasensitive, Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum Dot-Based Microfluidic Protein Chip
Hu, M; Yan, J; He, Y; Lu, HT; Weng, LX; Song, SP(宋世平); Fan, CH(樊春海); Wang, LH
2010
Source PublicationACS NANO
ISSN1936-0851
Volume4Issue:1Pages:488
AbstractSensitive and selective detection for cancer biomarkers are critical in cancer clinical-diagnostics. Here we developed a microfluidic protein chip for an ultrasensitive and multiplexed assay of cancer biomarkers. Aqueous-phase-synthesized CdTe/CdS quantum dots (aqQDs) were employed as fluorescent signal amplifiers to improve the detection sensitivity. Secondary antibodies (goat anti-mouse IgG) were conjugated to luminescent CdTe/CdS QDs to realize a versatile fluorescent probe that could be used for multiplexed detection in both sandwich and reverse phase immunoassays. We found that our microfluidic protein chip not only possessed ultrahigh femtomolar sensitivity for cancer biomarkers, but was selective enough to be directly used in serum. This protein chip thus combines the high-throughput capabilities of a microfluidic network with the high sensitivity and multicolor imaging ability offered by highly fluorescent QDs, which can become a promising diagnostic tool In clinical applications.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000273863400060
Citation statistics
Cited Times:195[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13063
Collection中科院上海应用物理研究所2004-2010年
Recommended Citation
GB/T 7714
Hu, M,Yan, J,He, Y,et al. Ultrasensitive, Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum Dot-Based Microfluidic Protein Chip[J]. ACS NANO,2010,4(1):488.
APA Hu, M.,Yan, J.,He, Y.,Lu, HT.,Weng, LX.,...&Wang, LH.(2010).Ultrasensitive, Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum Dot-Based Microfluidic Protein Chip.ACS NANO,4(1),488.
MLA Hu, M,et al."Ultrasensitive, Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum Dot-Based Microfluidic Protein Chip".ACS NANO 4.1(2010):488.
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