CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A G-Quadruplex/Hemin Complex with Switchable Peroxidase Activity by DNA Hybridization
Shao, CY; Lu, N(鲁娜); Sun, DM
2012
Source PublicationCHINESE JOURNAL OF CHEMISTRY
ISSN1001-604X
Volume30Issue:7
AbstractA hemin-binding DNA G-quadruplex (also known as a hemin aptamer or DNAzyme) has been previously reported to be able to enhance the peroxidase activity of hemin. In this work, we described a DNAzyme structure that had an effector-recognizing part appearing as a single stranded DNA linkage flanked by two split G-quadruplex halves. Hybridization of the single stranded part in the enzyme with a perfectly matched DNA strand (effector) formed a rigid DNA duplex between the two G-quadruplex halves and thus efficiently suppressed the enzymatic activity of the G-quadruplex/hemin complex, while the mismatched effector strand was not able to regulate the peroxidase activity effectively. With 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) as an oxidizable substrate, we were able to characterize the formation of the re-engineered G-quadruplex/hemin complex and verify its switchable peroxidase activity. Our results show that the split G-quadruplex is an especially useful module to design low-cost and label-free sensors toward various biologically or environmentally interesting targets.
Language英语
Funding Project应物所项目组
WOS IDWOS:000306562300035
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13073
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Shao, CY,Lu, N,Sun, DM. A G-Quadruplex/Hemin Complex with Switchable Peroxidase Activity by DNA Hybridization[J]. CHINESE JOURNAL OF CHEMISTRY,2012,30(7).
APA Shao, CY,Lu, N,&Sun, DM.(2012).A G-Quadruplex/Hemin Complex with Switchable Peroxidase Activity by DNA Hybridization.CHINESE JOURNAL OF CHEMISTRY,30(7).
MLA Shao, CY,et al."A G-Quadruplex/Hemin Complex with Switchable Peroxidase Activity by DNA Hybridization".CHINESE JOURNAL OF CHEMISTRY 30.7(2012).
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