Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin | |
| Zheng, XX; Wen, YQ; Zhang, J（张娟）; Wang, LH（王丽华）; Song, SP（宋世平）; Zhang, H; Fan, CH（樊春海） | |
| 2012 | |
| Source Publication | NUCLEAR SCIENCE AND TECHNIQUES
 |
| ISSN | 1001-8042 |
| Volume | 23Issue:5 |
| Abstract | Resonance light scattering (RLS) is a sensitive technique for monitoring scattered light induced by extended aggregates of chromophores. It has been widely used to study aggregations for its simple manipulation, high sensitivity and great versatility. Gold nanoparticles generate colorful light-scattering signals due to their unique surface plasmon resonances, hence extraordinary light scattering upon aggregation. In this paper we report a rapid and sensitive method based on gold nanoparticles and DNA aptamer to detect protein biomarkers by RLS. Thiol modified thrombin aptamer was covalently assembled to the surface of gold nanoparticles as nanobio probes. As thrombin has two specific binding sites for its aptamer, it can bridge the well dispersed nanoparticles and lead to a network of particle aggregations. The formation of aggregation ia measured by RLS, and the specific detection of thrombin at nM level is achieved. The method has good specificity. |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000314013200011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13077 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Zheng, XX,Wen, YQ,Zhang, J,et al. A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(5). |
| APA | Zheng, XX.,Wen, YQ.,Zhang, J.,Wang, LH.,Song, SP.,...&Fan, CH.(2012).A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin.NUCLEAR SCIENCE AND TECHNIQUES,23(5). |
| MLA | Zheng, XX,et al."A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin".NUCLEAR SCIENCE AND TECHNIQUES 23.5(2012). |
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