CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin
Zheng, XX; Wen, YQ; Zhang, J(张娟); Wang, LH(王丽华); Song, SP(宋世平); Zhang, H; Fan, CH(樊春海)
2012
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
Volume23Issue:5
AbstractResonance light scattering (RLS) is a sensitive technique for monitoring scattered light induced by extended aggregates of chromophores. It has been widely used to study aggregations for its simple manipulation, high sensitivity and great versatility. Gold nanoparticles generate colorful light-scattering signals due to their unique surface plasmon resonances, hence extraordinary light scattering upon aggregation. In this paper we report a rapid and sensitive method based on gold nanoparticles and DNA aptamer to detect protein biomarkers by RLS. Thiol modified thrombin aptamer was covalently assembled to the surface of gold nanoparticles as nanobio probes. As thrombin has two specific binding sites for its aptamer, it can bridge the well dispersed nanoparticles and lead to a network of particle aggregations. The formation of aggregation ia measured by RLS, and the specific detection of thrombin at nM level is achieved. The method has good specificity.
Language英语
Funding Project应物所项目组
WOS IDWOS:000314013200011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13077
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zheng, XX,Wen, YQ,Zhang, J,et al. A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(5).
APA Zheng, XX.,Wen, YQ.,Zhang, J.,Wang, LH.,Song, SP.,...&Fan, CH.(2012).A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin.NUCLEAR SCIENCE AND TECHNIQUES,23(5).
MLA Zheng, XX,et al."A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin".NUCLEAR SCIENCE AND TECHNIQUES 23.5(2012).
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