A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin

CAS OpenIR > 中科院上海应用物理研究所2011-2020年

	A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin
	Zheng, XX; Wen, YQ; Zhang, J（张娟）; Wang, LH（王丽华）; Song, SP（宋世平）; Zhang, H; Fan, CH（樊春海）
	2012
Source Publication	NUCLEAR SCIENCE AND TECHNIQUES
ISSN	1001-8042
Volume	23 Issue:5
Abstract	Resonance light scattering (RLS) is a sensitive technique for monitoring scattered light induced by extended aggregates of chromophores. It has been widely used to study aggregations for its simple manipulation, high sensitivity and great versatility. Gold nanoparticles generate colorful light-scattering signals due to their unique surface plasmon resonances, hence extraordinary light scattering upon aggregation. In this paper we report a rapid and sensitive method based on gold nanoparticles and DNA aptamer to detect protein biomarkers by RLS. Thiol modified thrombin aptamer was covalently assembled to the surface of gold nanoparticles as nanobio probes. As thrombin has two specific binding sites for its aptamer, it can bridge the well dispersed nanoparticles and lead to a network of particle aggregations. The formation of aggregation ia measured by RLS, and the specific detection of thrombin at nM level is achieved. The method has good specificity.
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000314013200011
Citation statistics	Cited Times:7[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/13077
Collection	中科院上海应用物理研究所2011-2020年
Recommended Citation GB/T 7714	Zheng, XX,Wen, YQ,Zhang, J,et al. A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(5).
APA	Zheng, XX.,Wen, YQ.,Zhang, J.,Wang, LH.,Song, SP.,...&Fan, CH.(2012).A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin.NUCLEAR SCIENCE AND TECHNIQUES,23(5).
MLA	Zheng, XX,et al."A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin".NUCLEAR SCIENCE AND TECHNIQUES 23.5(2012).

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