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Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates
Pei, H; Li, F(李凡); Wan, Y; Wei, M(魏敏); Liu, HJ; Su, Y; Chen, N(陈楠); Huang, Q(黄庆); Fan, CH(樊春海)
2012
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume134Issue:29
AbstractConjugates of DNA and gold nanoparticles (AuNPs) typically exploit the strong Au-S chemistry to self-assemble thiolated oligonucleotides at AuNPs. However, it remains challenging to precisely control the orientation and conformation of surface tethered oligonucleotides and finely tune the hybridization ability. We herein report a novel strategy for spatially controlled functionalization of AuNPs with designed diblock oligonucleotides that are free of modifications. We have demonstrated that poly adenine (polyA) can serve as an effective anchoring block for preferential binding with the AuNP surface, and the appended recognition block adopts an upright conformation that favors DNA hybridization. The lateral spacing and surface density of DNA on AuNPs can also be systematically modulated by adjusting the length of the polyA block. Significantly, this diblock oligonucleotide strategy results in DNA-AuNPs nano conjugates with high and tunable hybridization ability, which form the basis of a rapid plasmonic DNA sensor.
Language英语
Funding Project应物所项目组
WOS IDWOS:000306724500013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13127
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Pei, H,Li, F,Wan, Y,et al. Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(29).
APA Pei, H.,Li, F.,Wan, Y.,Wei, M.,Liu, HJ.,...&Fan, CH.(2012).Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,134(29).
MLA Pei, H,et al."Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134.29(2012).
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