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| Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates | |
| Pei, H; Li, F（李凡）; Wan, Y; Wei, M（魏敏）; Liu, HJ; Su, Y; Chen, N（陈楠）; Huang, Q（黄庆）; Fan, CH（樊春海） | |
| 2012 | |
| Source Publication | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| ISSN | 0002-7863 |
| Volume | 134Issue:29 |
| Abstract | Conjugates of DNA and gold nanoparticles (AuNPs) typically exploit the strong Au-S chemistry to self-assemble thiolated oligonucleotides at AuNPs. However, it remains challenging to precisely control the orientation and conformation of surface tethered oligonucleotides and finely tune the hybridization ability. We herein report a novel strategy for spatially controlled functionalization of AuNPs with designed diblock oligonucleotides that are free of modifications. We have demonstrated that poly adenine (polyA) can serve as an effective anchoring block for preferential binding with the AuNP surface, and the appended recognition block adopts an upright conformation that favors DNA hybridization. The lateral spacing and surface density of DNA on AuNPs can also be systematically modulated by adjusting the length of the polyA block. Significantly, this diblock oligonucleotide strategy results in DNA-AuNPs nano conjugates with high and tunable hybridization ability, which form the basis of a rapid plasmonic DNA sensor. |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000306724500013 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13127 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Pei, H,Li, F,Wan, Y,et al. Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(29). |
| APA | Pei, H.,Li, F.,Wan, Y.,Wei, M.,Liu, HJ.,...&Fan, CH.(2012).Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,134(29). |
| MLA | Pei, H,et al."Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA-Gold Nanoparticle Nanoconjugates".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134.29(2012). |
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| File Name/Size | DocType | Version | Access | License | ||
| Designed Diblock Oli（989KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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