CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA
Wen, YL; Pei, H; Shen, Y; Xi, JJ; Lin, MH; Lu, N(鲁娜); Shen, XZ; Li, J; Fan, CH(樊春海)
2012
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume2
AbstractMicroRNAs (miRNAs) have been identified as promising cancer biomarkers due to their stable presence in serum. As an alternative to PCR-based homogenous assays, surface-based electrochemical biosensors offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. Nevertheless, the sensitivity of miRNA sensors is often limited by mass transport and crowding effects at the water-electrode interface. To address such challenges, we herein report a DNA nanostructure-based interfacial engineering approach to enhance binding recognition at the gold electrode surface and drastically improve the detection sensitivity. By employing this novel strategy, we can directly detect as few as attomolar (< 1, 000 copies) miRNAs with high single-base discrimination ability. Given that this ultrasensitive electrochemical miRNA sensor (EMRS) is highly reproducible and essentially free of prior target labeling and PCR amplification, we also demonstrate its application by analyzing miRNA expression levels in clinical samples from esophageal squamous cell carcinoma (ESCC) patients.
Language英语
Funding Project应物所项目组
WOS IDWOS:000311348900001
Citation statistics
Cited Times:142[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13136
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Wen, YL,Pei, H,Shen, Y,et al. DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA[J]. SCIENTIFIC REPORTS,2012,2.
APA Wen, YL.,Pei, H.,Shen, Y.,Xi, JJ.,Lin, MH.,...&Fan, CH.(2012).DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA.SCIENTIFIC REPORTS,2.
MLA Wen, YL,et al."DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA".SCIENTIFIC REPORTS 2(2012).
Files in This Item: Download All
File Name/Size DocType Version Access License
DNA Nanostructure-ba(598KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wen, YL]'s Articles
[Pei, H]'s Articles
[Shen, Y]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wen, YL]'s Articles
[Pei, H]'s Articles
[Shen, Y]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wen, YL]'s Articles
[Pei, H]'s Articles
[Shen, Y]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.