CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I
Chen, JJ; Zhang, C; Zhao, BY; Mi, LJ(米丽娟); Zhang, Y(张益); Hu, J(胡钧)
2012
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
Volume23Issue:4
AbstractFluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/sensing technologies. In this paper, we report that the fluorescence of the double-stranded DNA (dsDNA) and SYBR Green I (SG) system (dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe2O3@DMSA) and gold nanoparticles in suitable concentrations, but positively charged nanoparticles quench the fluorescence. Effects of the Fe2O3@DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities. The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA. However, the dsDNA concentration determines the fluorescence amplitude. It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG. The finding may be useful in sensitive biomolecular detection.
Language英语
Funding Project应物所项目组
WOS IDWOS:000310092200011
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13143
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Chen, JJ,Zhang, C,Zhao, BY,et al. Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(4).
APA Chen, JJ,Zhang, C,Zhao, BY,Mi, LJ,Zhang, Y,&Hu, J.(2012).Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I.NUCLEAR SCIENCE AND TECHNIQUES,23(4).
MLA Chen, JJ,et al."Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I".NUCLEAR SCIENCE AND TECHNIQUES 23.4(2012).
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