CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Genetic analysis with nanoPCR
Pan, D(潘敦); Mi, LJ(米丽娟); Huang, Q(黄庆); Hu, J(胡钧); Fan, CH(樊春海)
AbstractPolymerase chain reaction (PCR) has become a standard and important molecular biological technique with numerous applications in genetic analysis, forensics and in vitro diagnostics. Since its invention in the 1980s, there has been dramatic performance improvement arising from long-lasting efforts to optimize amplification conditions in both academic studies and commercial applications. More recently, a range of nanometer-sized materials including metal nanoparticles, semiconductor quantum dots, carbon nanomaterials and polymer nanoparticles, have shown unique effects in tuning amplification processes of PCR. It is proposed that these artificial nanomaterials mimic protein components in the natural DNA replication machinery in vivo. These so-called nanomaterials-assisted PCR (nanoPCR) strategies shed new light on powerful PCR with unprecedented sensitivity, selectivity and extension rate. In this review, we aim to summarize recent progress in this direction and discuss possible mechanisms for such performance improvement and potential applications in genetic analysis (particularly gene typing and haplotyping) and diagnostics.
Funding Project应物所项目组
WOS IDWOS:000309127600001
Citation statistics
Document Type期刊论文
Recommended Citation
GB/T 7714
Pan, D,Mi, LJ,Huang, Q,et al. Genetic analysis with nanoPCR[J]. INTEGRATIVE BIOLOGY,2012,4(10).
APA Pan, D,Mi, LJ,Huang, Q,Hu, J,&Fan, CH.(2012).Genetic analysis with nanoPCR.INTEGRATIVE BIOLOGY,4(10).
MLA Pan, D,et al."Genetic analysis with nanoPCR".INTEGRATIVE BIOLOGY 4.10(2012).
Files in This Item: Download All
File Name/Size DocType Version Access License
Genetic analysis wit(2736KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Pan, D(潘敦)]'s Articles
[Mi, LJ(米丽娟)]'s Articles
[Huang, Q(黄庆)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Pan, D(潘敦)]'s Articles
[Mi, LJ(米丽娟)]'s Articles
[Huang, Q(黄庆)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Pan, D(潘敦)]'s Articles
[Mi, LJ(米丽娟)]'s Articles
[Huang, Q(黄庆)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Genetic analysis with nanoPCR.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.