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Heteroepitaxial Streptavidin Nanocrystals Reveal Critical Role of Proton "Fingers" and Subsurface Atoms in Determining Adsorbed Protein Orientation
Czajkowsky, DM; Li, L((李林); Sun, JL; Hu, J(胡钧); Shao, ZF
2012
Source PublicationACS NANO
ISSN1936-0851
Volume6Issue:1
AbstractCharacterization of noncovalent interactions between nanometer-sized structures, such as proteins, and solid surfaces is a subject of intense interest of late owing to the rapid development of numerous solid materials for medical and technological applications. Yet the rational design of these surfaces to promote the adsorption of specific nanoscale complexes is hindered by a lack of an understanding of the noncovalent interactions between nanostructures and solid surfaces. Here we take advantage of the unexpected observation of two-dimensional nanocrystals of streptavidin on muscovite mica to provide details of the streptavidin mica interface. Analysis of atomic force microscopic Images together with structural modeling identifies six positively charged residues whose terminal amine locations match the positions of the single atom-sized anionic cavities in the basal mica surface to within 1 A. Moreover, we find that the streptavidin crystallites are oriented only along a single direction on this surface and not in either of three different directions as they must be if the protein Interacted solely with the 3-fold symmetric basal surface atoms. Hence, this broken symmetry indicates that the terminal amine protons must also interact directly with the subsurface hydroxide atoms that line the bottom of these anionic cavities and generate only a single axis of symmetry. Thus, in total, these results reveal that subsurface atoms can have a significant influence on protein adsorption and orientation and identify the insertion of proton "fingers" as a means by which proteins may generally interact with solid surfaces.
Language英语
Funding Project应物所项目组
WOS IDWOS:000299368300024
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13181
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Czajkowsky, DM,Li, L,Sun, JL,et al. Heteroepitaxial Streptavidin Nanocrystals Reveal Critical Role of Proton "Fingers" and Subsurface Atoms in Determining Adsorbed Protein Orientation[J]. ACS NANO,2012,6(1).
APA Czajkowsky, DM,Li, L,Sun, JL,Hu, J,&Shao, ZF.(2012).Heteroepitaxial Streptavidin Nanocrystals Reveal Critical Role of Proton "Fingers" and Subsurface Atoms in Determining Adsorbed Protein Orientation.ACS NANO,6(1).
MLA Czajkowsky, DM,et al."Heteroepitaxial Streptavidin Nanocrystals Reveal Critical Role of Proton "Fingers" and Subsurface Atoms in Determining Adsorbed Protein Orientation".ACS NANO 6.1(2012).
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