CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Kinetic Parameters Estimation in the Polymerase Chain Reaction Process Using the Genetic Algorithm
Li, LT(李兰婷); Wang, C; Song, B(宋波); Mi, LJ(米丽娟); Hu, J(胡钧)
2012
Source PublicationINDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
ISSN0888-5885
Volume51Issue:40
AbstractAnalyzing the polymerase chain reaction (PCR) process by mathematical modelling is of importance in terms of uncovering the dynamic mechanism of PCR and predicting DNA amplification performance. Construction of an ideal PCR model, however, requires sufficient and accurate kinetic parameters, which cannot be easily obtained directly from experimental data. In this work, a genetic algorithm-based approach was introduced for optimizing kinetic parameters of PCR such as the rate constant of polymerase catalyst reaction. The fitted model agrees well with experimental data and predicts the DNA amplification yields as a function of cycle number. The kinetic parameters are not dependent on initial concentrations or the fragment types of DNA amplification. The model also allows us to predict the threshold cycle in real-time PCR, which is helpful for estimating the initial amount of DNA template and determining the optimal PCR reaction conditions.
Language英语
Funding Project应物所项目组
WOS IDWOS:000309624500031
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13204
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Li, LT,Wang, C,Song, B,et al. Kinetic Parameters Estimation in the Polymerase Chain Reaction Process Using the Genetic Algorithm[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2012,51(40).
APA Li, LT,Wang, C,Song, B,Mi, LJ,&Hu, J.(2012).Kinetic Parameters Estimation in the Polymerase Chain Reaction Process Using the Genetic Algorithm.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,51(40).
MLA Li, LT,et al."Kinetic Parameters Estimation in the Polymerase Chain Reaction Process Using the Genetic Algorithm".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 51.40(2012).
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