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Local and global sensitivity analysis of PCR kinetic model
Li, LT(李兰婷); Mi, LJ(米丽娟); Hu, J(胡钧)
2012
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
Volume23Issue:2
AbstractA mathematical model of polymerase chain reaction (PCR) containing uncertain and time-varying parameters has become important for model-based guidance experiment design. In this study, the local and global sensitivity analyses were conducted to identify that the responses of PCR process vary with their parameters of initial reactant concentrations and rate constants. Our results showed that the template concentration in initial reactant concentrations had the largest effect on DNA amplification yield. The rate constant characteristics showed that the local sensitivity basically determined the specific reactions; and the global sensitivity, the non-specific reactions. Our work should be helpful for optimizing PCR experimental conditions, and determining the PCR parameter sensitivities.
Language英语
Funding Project应物所项目组
WOS IDWOS:000303784600011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13210
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, LT,Mi, LJ,Hu, J. Local and global sensitivity analysis of PCR kinetic model[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(2).
APA Li, LT,Mi, LJ,&Hu, J.(2012).Local and global sensitivity analysis of PCR kinetic model.NUCLEAR SCIENCE AND TECHNIQUES,23(2).
MLA Li, LT,et al."Local and global sensitivity analysis of PCR kinetic model".NUCLEAR SCIENCE AND TECHNIQUES 23.2(2012).
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