Nano Rolling-Circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis

CAS OpenIR > 中科院上海应用物理研究所2011-2019年

	Nano Rolling-Circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis
	Yan, J（颜娟）; Su, S; He, SJ; He, Y; Zhao, B; Wang, DF; Zhang, HL; Huang, Q（黄庆）; Song, SP（宋世平）; Fan, C（樊春海）
	2012
Source Publication	ANALYTICAL CHEMISTRY
ISSN	0003-2700
Volume	84 Issue:21
Abstract	Although "hot spots" have been proved to contribute to surface enhanced Raman scattering (SERS), less attention was paid to increase the number of the "hot spot" to directly enhance the Raman signals in bioanalytical systems. Here we report a new strategy based on nano rolling-circle amplification (nanoRCA) and nano hyperbranched rolling-circle amplification (nanoHRCA) to increase "hot spot" groups for protein microarrays. First, protein and ssDNA are coassembled on gold nanoparticles, making the assembled probe have both binding ability and hybridization ability. Second, the ssDNAs act as primers to initiate in situ RCA reaction to produced long ssDNAs. Third, a large number of SEAS probes are loaded on the long ssDNA templetes, allowing thousands of SERS probes involved in each biomolecular recognition event. The strategy offered high-efficiency Raman enhancement and could detect less than 10 zeptomolar protein molecules in protein rnicroarray analysis.
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000310664600039
Citation statistics	Cited Times:43[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/13221
Collection	中科院上海应用物理研究所2011-2019年
Recommended Citation GB/T 7714	Yan, J,Su, S,He, SJ,et al. Nano Rolling-Circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis[J]. ANALYTICAL CHEMISTRY,2012,84(21).
APA	Yan, J.,Su, S.,He, SJ.,He, Y.,Zhao, B.,...&Fan, C.(2012).Nano Rolling-Circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis.ANALYTICAL CHEMISTRY,84(21).
MLA	Yan, J,et al."Nano Rolling-Circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis".ANALYTICAL CHEMISTRY 84.21(2012).

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