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Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction
Wang, P(王萍); Ge, ZL(葛志磊); Pei, H; Wang, LH(王丽华); Fan, CH(樊春海)
2012
Source PublicationACTA CHIMICA SINICA
ISSN0567-7351
Volume70Issue:20
AbstractThe hybridization chain reaction (HCR), a type of nucleic acid amplification method, was studied by a quartz crystal resonator technique. We probed the properties of the boundary DNA layer with a thickness of the viscous penetration depth at the crystal-solution interface. According to the relationship between the change of resonant frequency and the mass adsorbed on the crystal surface, the kinetics of surface-initiated HCR was measured, as well as the viscosity and the shear modulus of H1/H2 solution with different concentrations near a solid substrate. This work provides a new method for studying nucleic acid reactions at solid surface and mechanistic insight for HCR-based amplification and biodetection.
Language英语
Funding Project应物所项目组
WOS IDWOS:000311533600004
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13269
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Wang, P,Ge, ZL,Pei, H,et al. Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction[J]. ACTA CHIMICA SINICA,2012,70(20).
APA Wang, P,Ge, ZL,Pei, H,Wang, LH,&Fan, CH.(2012).Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction.ACTA CHIMICA SINICA,70(20).
MLA Wang, P,et al."Quartz Crystal Microbalance Studies on Surface-Initiated DNA Hybridization Chain Reaction".ACTA CHIMICA SINICA 70.20(2012).
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