CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads
Tang, RB; Chai, WM; Yang, GY; Xie, HL(谢红兰); Chen, KM
2012
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:9
AbstractSuper-paramagnetic microbeads are widely used for cell isolation. Evaluation of the binding affinity of microbeads to cells using optical microscopy has been limited by its small scope. Here, magnetic property of microbeads was first investigated by using synchrotron radiation (SR) in-line x-ray phase contrast imaging (PCI). The cell line mouse LLC (Lewis lung carcinoma) was selected for cell adhesion studies. Targeted microbeads were prepared by attaching anti-VEGFR2 (vascular endothelial growth factor receptor-2) antibody to the shell of the microbeads. The bound microbeads were found to better adhere to LLC cells than unbound ones. PCI dynamically and clearly showed the magnetization and demagnetization of microbeads in PE-50 tube. The cells incubated with different types of microbeads were imaged by PCI, which provided clear and real-time visualization of the cell isolation. Therefore, PCI might be considered as a novel and efficient tool for further cell isolation studies.
Language英语
Funding Project应物所项目组
WOS IDWOS:000309554700048
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13345
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Tang, RB,Chai, WM,Yang, GY,et al. X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads[J]. PLOS ONE,2012,7(9).
APA Tang, RB,Chai, WM,Yang, GY,Xie, HL,&Chen, KM.(2012).X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads.PLOS ONE,7(9).
MLA Tang, RB,et al."X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads".PLOS ONE 7.9(2012).
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