CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A graphene-based platform for fluorescent detection of SNPs
Xu, Hui; Yang, Qing; Li, Fan; Tang, Linsheng; Gao, Shanmin; Jiang, Bowei; Zhao, Xingchun; Wang, Lihua(王丽华); Fan, Chunhai(樊春海)
2013
Source PublicationANALYST
ISSN0003-2654
Volume138Issue:9Pages:CONCATENATE(Sheet1!I9,-Sheet1!J9)
AbstractA novel fluorescent single nucleotide polymorphism (SNP) assay was developed by using Graphene Oxide (GO), which provides a fast, sensitive and simple method for SNP detection. The strategy was based on the single base extension reaction and different absorption capacity of fluorescein labeled dGTP (dGTP-Fl) and double-stranded DNA (dsDNA) to GO. dGTP-Fl is incorporated into the probe by extension reaction for the mutant target but not for the wild target, which leads to recovered fluorescence for the mutant target because of weak interaction between dsDNA and GO and weak fluorescence for the wild target because of the quenched fluorescence of dGTP-Fl by GO. The method shows a linear range for the mutant-type target from 3 nM to 50 nM and 3 nM is the detection limit. It was noted that as low as 10% mutant-type target could be detected in the presence of the wild-type target, in which the concentration is 9 times higher than that of the mutant-type target.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000316959800025
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13596
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Xu, Hui,Yang, Qing,Li, Fan,et al. A graphene-based platform for fluorescent detection of SNPs[J]. ANALYST,2013,138(9):CONCATENATE(Sheet1!I9,-Sheet1!J9).
APA Xu, Hui.,Yang, Qing.,Li, Fan.,Tang, Linsheng.,Gao, Shanmin.,...&Fan, Chunhai.(2013).A graphene-based platform for fluorescent detection of SNPs.ANALYST,138(9),CONCATENATE(Sheet1!I9,-Sheet1!J9).
MLA Xu, Hui,et al."A graphene-based platform for fluorescent detection of SNPs".ANALYST 138.9(2013):CONCATENATE(Sheet1!I9,-Sheet1!J9).
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