CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Gold nanoparticle-assisted primer walking for closing the human chromosomal gap
Li, Haikuo; Shi, Baochen; Li, Xueling; Nyegaard, Steffen; Lu, Ming; Lu, Junhong; Cao, Xueyan; Huang, Qing(黄庆); Fan, Chunhai(樊春海); Chen, Runsheng; Hu, Jun(胡钧)
2013
Source PublicationANALYTICAL METHODS
ISSN1759-9660
Volume5Issue:18Pages:CONCATENATE(Sheet1!I139,-Sheet1!J139)
AbstractThe finished sequence of the human genome still contains 260 euchromatic gaps. All the PCR-based genome walking techniques used to close gaps have common limitations, such as low efficiency and low specificity. We herein describe a strategy to solve this problem by employing gold nanoparticles (AuNPs) to improve the efficiency in primer walking amplification. We used this strategy to close a gap in human chromosome 5 containing a DNA stretch composed of the 12SAT repeat. The obtained gap sequence is highly conserved among several mammalian genomes. The demonstrated AuNP-assisted primer walking strategy is capable of effectively improving the specificity of PCR amplification and enriching the yield of target DNA fragments; it offers a new avenue for closing gaps left by current sequencing methods.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000323518200029
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13618
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, Haikuo,Shi, Baochen,Li, Xueling,et al. Gold nanoparticle-assisted primer walking for closing the human chromosomal gap[J]. ANALYTICAL METHODS,2013,5(18):CONCATENATE(Sheet1!I139,-Sheet1!J139).
APA Li, Haikuo.,Shi, Baochen.,Li, Xueling.,Nyegaard, Steffen.,Lu, Ming.,...&Hu, Jun.(2013).Gold nanoparticle-assisted primer walking for closing the human chromosomal gap.ANALYTICAL METHODS,5(18),CONCATENATE(Sheet1!I139,-Sheet1!J139).
MLA Li, Haikuo,et al."Gold nanoparticle-assisted primer walking for closing the human chromosomal gap".ANALYTICAL METHODS 5.18(2013):CONCATENATE(Sheet1!I139,-Sheet1!J139).
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