Hierarchical ordering of amyloid fibrils on the mica surface

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	Hierarchical ordering of amyloid fibrils on the mica surface
	Zhou, Xingfei; Zhang, Yingying; Zhang, Feng; Pillai, Saju; Liu, Jianhua; Li, Rong; Dai, Bin; Li, Bin（李宾）; Zhang, Yi（张益）
	2013
Source Publication	NANOSCALE
ISSN	2040-3364
Volume	5 Issue:11 Pages:CONCATENATE(Sheet1!I145,-Sheet1!J145)
Abstract	The aggregation of amyloid peptides into ordered fibrils is closely associated with many neuro-degenerative diseases. The surfaces of cell membranes and biomolecules are believed to play important roles in modulation of peptide aggregation under physiological conditions. Experimental studies of fibrillogenesis at the molecular level in vivo, however, are inherently challenging, and the molecular mechanisms of how surface affects the structure and ordering of amyloid fibrils still remain elusive. Herein we have investigated the aggregation behavior of insulin peptides within water films adsorbed on the mica surface. AFM measurements revealed that the structure and orientation of fibrils were significantly affected by the mica lattice and the peptide concentration. At low peptide concentration (similar to 0.05 mg mL(-1)), there appeared a single layer of short and well oriented fibrils with a mean height of 1.6 nm. With an increase of concentration to a range of 0.2-2.0 mg mL(-1), a different type of fibrils with a mean height of 3.8 nm was present. Interestingly, when the concentration was above 2.0 mg mL(-1), the thicker fibrils exhibited two-dimensional liquid-crystal-like ordering probably caused by the combination of entropic and electrostatic forces. These results could help us gain better insight into the effects of the substrate on amyloid fibrillation.
Indexed By	SCI
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000319008700033
Citation statistics
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/13619
Collection	中科院上海应用物理研究所2011-2020年
Recommended Citation GB/T 7714	Zhou, Xingfei,Zhang, Yingying,Zhang, Feng,et al. Hierarchical ordering of amyloid fibrils on the mica surface[J]. NANOSCALE,2013,5(11):CONCATENATE(Sheet1!I145,-Sheet1!J145).
APA	Zhou, Xingfei.,Zhang, Yingying.,Zhang, Feng.,Pillai, Saju.,Liu, Jianhua.,...&Zhang, Yi.(2013).Hierarchical ordering of amyloid fibrils on the mica surface.NANOSCALE,5(11),CONCATENATE(Sheet1!I145,-Sheet1!J145).
MLA	Zhou, Xingfei,et al."Hierarchical ordering of amyloid fibrils on the mica surface".NANOSCALE 5.11(2013):CONCATENATE(Sheet1!I145,-Sheet1!J145).

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