CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells
Feng Bianying; Guo Linjie; Wang Lihua(王丽华); Li Fan; Lu Jianxin; Gao Jimin; Fan Chunhai(樊春海); Huang Qing(黄庆)
2013
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume85Issue:16Pages:CONCATENATE(Sheet1!I8,-Sheet1!J8)
AbstractAnalysis of peptide-receptor interactions provides insights for understanding functions of proteins in cells. In this work, we report the development of a fluorescent biosensor for the analysis of peptide-receptor interactions using graphene oxide (GO) and fluorescein isothiocyanate (FITC)-labeled octreotide (FOC). Octreotide is a synthesized cyclic peptide with somatostatin-like bioactivity that has been clinically employed. FOC exhibits high adsorption affinity for GO, and its binding results in efficient fluorescence quenching of FITC. Interestingly, the specific binding of the antibody anti-octreotide (AOC) with FOC competitively releases FOC from the GO surface, leading to the recovery of fluorescence. By using this GO-based fluorescent platform, we can detect AOC with a low detection limit of 2 ng/mL. As a step further, we employ this GO-FOC biosensor to image somatostatin receptor subtype 2 overexpressed AR42J tumor cells, which demonstrates high promise for molecular imaging in cancer diagnosis.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000323471800018
Citation statistics
Cited Times:57[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13652
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Feng Bianying,Guo Linjie,Wang Lihua,et al. A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells[J]. ANALYTICAL CHEMISTRY,2013,85(16):CONCATENATE(Sheet1!I8,-Sheet1!J8).
APA Feng Bianying.,Guo Linjie.,Wang Lihua.,Li Fan.,Lu Jianxin.,...&Huang Qing.(2013).A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells.ANALYTICAL CHEMISTRY,85(16),CONCATENATE(Sheet1!I8,-Sheet1!J8).
MLA Feng Bianying,et al."A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells".ANALYTICAL CHEMISTRY 85.16(2013):CONCATENATE(Sheet1!I8,-Sheet1!J8).
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