Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine | |
| Li, Shengxi; Zhang, Jiong; Deng, Wangping; Shen, Xizhong; Song, Shiping（宋世平）; Fan, Chunhai(樊春海） | |
| 2013 | |
| Source Publication | ELECTROANALYSIS
 |
| ISSN | 1040-0397 |
| Volume | 25Issue:4Pages:CONCATENATE(Sheet1!I10,-Sheet1!J10) |
| Abstract | We report a highly sensitive competitive-type assay for multiplexing electrochemical detection of clenbuterol in pig urine using a 16-sensor array. In this design, the clenbuterol was first conjugated with bovine serum albumin (BSA), and then the conjugates were immobilized on the electrode surface to compete with the free clenbuterol in the sample for specific antibody. Under the optimal conditions, the reproducibility of the clenbuterol electrochemical immunosensor was evaluated to be 3.4% (coefficient of variation, CV) and the limit of detection was estimated to be 1.3pg/mL. The very low detection limit was probably derived from the higher efficiency of the competitive immunoreaction caused by appropriate quantities of the clenbuterol immobilized on the 16-sensor array and the suitable amount of anti-clenbuterol antibody in the assay system. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000317677400009 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13653 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Li, Shengxi,Zhang, Jiong,Deng, Wangping,et al. A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine[J]. ELECTROANALYSIS,2013,25(4):CONCATENATE(Sheet1!I10,-Sheet1!J10). |
| APA | Li, Shengxi,Zhang, Jiong,Deng, Wangping,Shen, Xizhong,Song, Shiping,&Fan, Chunhai.(2013).A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine.ELECTROANALYSIS,25(4),CONCATENATE(Sheet1!I10,-Sheet1!J10). |
| MLA | Li, Shengxi,et al."A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine".ELECTROANALYSIS 25.4(2013):CONCATENATE(Sheet1!I10,-Sheet1!J10). |
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| File Name/Size | DocType | Version | Access | License | ||
| A Highly Sensitive A（478KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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