CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine
Li, Shengxi; Zhang, Jiong; Deng, Wangping; Shen, Xizhong; Song, Shiping(宋世平); Fan, Chunhai(樊春海)
2013
Source PublicationELECTROANALYSIS
ISSN1040-0397
Volume25Issue:4Pages:CONCATENATE(Sheet1!I10,-Sheet1!J10)
AbstractWe report a highly sensitive competitive-type assay for multiplexing electrochemical detection of clenbuterol in pig urine using a 16-sensor array. In this design, the clenbuterol was first conjugated with bovine serum albumin (BSA), and then the conjugates were immobilized on the electrode surface to compete with the free clenbuterol in the sample for specific antibody. Under the optimal conditions, the reproducibility of the clenbuterol electrochemical immunosensor was evaluated to be 3.4% (coefficient of variation, CV) and the limit of detection was estimated to be 1.3pg/mL. The very low detection limit was probably derived from the higher efficiency of the competitive immunoreaction caused by appropriate quantities of the clenbuterol immobilized on the 16-sensor array and the suitable amount of anti-clenbuterol antibody in the assay system.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000317677400009
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13653
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, Shengxi,Zhang, Jiong,Deng, Wangping,et al. A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine[J]. ELECTROANALYSIS,2013,25(4):CONCATENATE(Sheet1!I10,-Sheet1!J10).
APA Li, Shengxi,Zhang, Jiong,Deng, Wangping,Shen, Xizhong,Song, Shiping,&Fan, Chunhai.(2013).A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine.ELECTROANALYSIS,25(4),CONCATENATE(Sheet1!I10,-Sheet1!J10).
MLA Li, Shengxi,et al."A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine".ELECTROANALYSIS 25.4(2013):CONCATENATE(Sheet1!I10,-Sheet1!J10).
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