CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy
Shen, Yi; Czajkowsky, Daniel M.; Li, Xiaowei; Sun, Jielin; Hu, Jun(胡钧); Shao, Zhifeng
2013
Source PublicationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
ISSN1533-4880
Volume13Issue:2Pages:CONCATENATE(Sheet1!I14,-Sheet1!J14)
AbstractSingle-molecule force spectroscopy has revolutionized our ability to probe the details of molecular structures and interactions, but the numbers of individual measurements required for achieving a statistically reliable result can sometimes prove daunting. To overcome this problem, a number of instruments have recently been developed that are capable of monitoring the behavior of tens of individual biomolecules simultaneously. In this work, we have constructed a novel electromagnetic apparatus for multiplex single molecule force measurements utilizing magnetic microspheres. In this system, the magnetic field is generated with an electron-lens of an electron microscope mated with a high voltage flash light circuit to rapidly attain a stable magnetic field. We show that this instrument can generate a uniform magnetic force of up to similar to 20 pN within 5 ms, over a region spanning 1 mm. The successful application of this apparatus to the force-dependent extension of dsDNA fully validates this approach. Furthermore, the lens-like design of the pole piece is fully compatible with optical imaging, thus allowing for the integration of single molecule fluorescence capabilities that should make this system a particularly powerful apparatus for multi-dimensional characterization of fast processes within interacting single molecules.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000318254500105
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13657
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Shen, Yi,Czajkowsky, Daniel M.,Li, Xiaowei,et al. A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2013,13(2):CONCATENATE(Sheet1!I14,-Sheet1!J14).
APA Shen, Yi,Czajkowsky, Daniel M.,Li, Xiaowei,Sun, Jielin,Hu, Jun,&Shao, Zhifeng.(2013).A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,13(2),CONCATENATE(Sheet1!I14,-Sheet1!J14).
MLA Shen, Yi,et al."A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 13.2(2013):CONCATENATE(Sheet1!I14,-Sheet1!J14).
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