CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors
Wan, Ying; Xu, Hui; Su, Yan; Zhu, Xinhua; Song, Shiping(宋世平); Fan, Chunhai(樊春海)
2013
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume41Pages:CONCATENATE(Sheet1!I22,-Sheet1!J22)
AbstractIn this work, we report a novel strategy of electrochemical DNA (E-DNA) sensor based on surface initiated enzymatic polymerization (SIEP). This DNA sensor employs a capture DNA probe labeled with thiol at its 3' terminal to be immobilized at gold electrode via gold-thiol chemistry. Oligo (ethylene glycol) -terminated thiols (SH-OEGs) are then used to prepare an oligonucleotide-incorporated nonfouling surface (ONS). After the sequence-specific recognition of target DNA, terminal deoxynucleotidyl transferase (TdT) is employed to catalyze the sequential addition of deoxynucleotides (dNTPs) at the 3'-OH group of target DNA without template. During the TdT-mediated extension reaction, by using biotinlated 2'-deoxyadenosine 5'-triphosphate (biotin-dATP), biotin labels are incorporated into the SIEP-generated long single-stranded DNA (ssDNA). Specific binding of avidin-horseradish peroxidase (Av-HRP) to the biotin label leads to enzyme turnover-based signal transduction. By using this new strategy, we demonstrated the high picomolar sensitivity and a broad detection range of six orders of magnitude. (C) 2012 Elsevier B.V. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000314191300078
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13662
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Wan, Ying,Xu, Hui,Su, Yan,et al. A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors[J]. BIOSENSORS & BIOELECTRONICS,2013,41:CONCATENATE(Sheet1!I22,-Sheet1!J22).
APA Wan, Ying,Xu, Hui,Su, Yan,Zhu, Xinhua,Song, Shiping,&Fan, Chunhai.(2013).A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors.BIOSENSORS & BIOELECTRONICS,41,CONCATENATE(Sheet1!I22,-Sheet1!J22).
MLA Wan, Ying,et al."A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors".BIOSENSORS & BIOELECTRONICS 41(2013):CONCATENATE(Sheet1!I22,-Sheet1!J22).
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