CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Analysis of the selectivity filter of the voltage-gated sodium channel NavRh
Zhang, Xu; Xia, Mengdie; Li, Yang; Liu, Huihui; Jiang, Xin; Ren, Wenlin; Wu, Jianping; DeCaen, Paul; Yu, Feng(郁锋); Huang, Sheng; He, Jianhua(何建华); Clapham, David E.; Yan, Nieng; Gong, Haipeng
2013
Source PublicationCELL RESEARCH
ISSN1001-0602
Volume23Issue:3Pages:CONCATENATE(Sheet1!I29,-Sheet1!J29)
AbstractNaChBac is a bacterial voltage-gated sodium (Na-v) channel that shows sequence similarity to voltage-gated calcium channels. To understand the ion-permeation mechanism of Na-v channels, we combined molecular dynamics simulation, structural biology and electrophysiological approaches to investigate the recently determined structure of NavRh, a marine bacterial NaChBac ortholog. Two Na+ binding sites are identified in the selectivity filter (SF) in our simulations: The extracellular Na+ ion first approaches site 1 constituted by the side groups of Ser181 and Glu183, and then spontaneously arrives at the energetically more favorable site 2 formed by the carbonyl oxygens of Leu179 and Thr178. In contrast, Ca2+ ions are prone to being trapped by Glu183 at site 1, which then blocks the entrance of both Na+ and Ca2+ to the vestibule of the SF. In addition, Na+ permeates through the selective filter in an asymmetrical manner, a feature that resembles that of the mammalian Na-v orthologs. The study reported here provides insights into the mechanism of ion selectivity on Na+ over Ca2+ in mammalian Na-v channels.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000316375000013
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13668
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zhang, Xu,Xia, Mengdie,Li, Yang,et al. Analysis of the selectivity filter of the voltage-gated sodium channel NavRh[J]. CELL RESEARCH,2013,23(3):CONCATENATE(Sheet1!I29,-Sheet1!J29).
APA Zhang, Xu.,Xia, Mengdie.,Li, Yang.,Liu, Huihui.,Jiang, Xin.,...&Gong, Haipeng.(2013).Analysis of the selectivity filter of the voltage-gated sodium channel NavRh.CELL RESEARCH,23(3),CONCATENATE(Sheet1!I29,-Sheet1!J29).
MLA Zhang, Xu,et al."Analysis of the selectivity filter of the voltage-gated sodium channel NavRh".CELL RESEARCH 23.3(2013):CONCATENATE(Sheet1!I29,-Sheet1!J29).
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