Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers | |
| Zhao, Bin; Yan, Juan; Wang, Dongfang; Ge, Zhilei; He, Shijiang; He, Dannong; Song, Shiping（宋世平）; Fan, Chunhai（樊春海） | |
| 2013 | |
| Source Publication | SMALL
 |
| ISSN | 1613-6810; 1613-6829 |
| Volume | 9Issue:15Pages:CONCATENATE(Sheet1!I47,-Sheet1!J47) |
| Abstract | There are still challenges for the development of multifunctional carbon nanotubes (CNTs). Here, a multiwalled carbon nanotube (MWCNT)-based rolling circle amplification system (CRCAS) is reported which allows in situ rolling circle replication of DNA primer on the surface of MWCNTs to create a long single-strand DNA (ssDNA) where a large number of nanoparticles or proteins could be loaded, forming a nano-biohybridized 3D structure with a powerful signal amplification ability. In this strategy, the binding ability of proteins, hybridization, replication ability of DNA, and the catalytical ability of enzymes are integrated on a single carbon nanotube. The CRCAS is then used to develop colorimetric and chemiluminescent assays for the highly sensitive and specific detection of cancer protein markers, alpha-fetoprotein (AFP) and prostate specific antigen (PSA). The colorimetric CRCAS assay is 4000 times more sensitive than a conventional enzyme-linked immunosorbent assay (ELISA), and its concentration range is 10 000 times wider. Control experiments show that as low as 10 pg mL(-1) AFP or PSA could be detected even in the presence of interfering protein markers with a more than 10(5)-fold greater concentration in the sample, demonstrating the high specificity of the CRCAS assay. The limit of detection of the chemiluminescent CRCAS assays for AFP and PSA are 5 fg mL(-1) (70 aM) and 10 fg mL(-1) (0.29 fM), respectively, indicating that the sensitivity is much higher than that of the colorimetric CRCAS assay. Importantly, CRCAS works well with real biological samples. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000327792600017 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13684 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Zhao, Bin,Yan, Juan,Wang, Dongfang,et al. Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers[J]. SMALL,2013,9(15):CONCATENATE(Sheet1!I47,-Sheet1!J47). |
| APA | Zhao, Bin.,Yan, Juan.,Wang, Dongfang.,Ge, Zhilei.,He, Shijiang.,...&Fan, Chunhai.(2013).Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers.SMALL,9(15),CONCATENATE(Sheet1!I47,-Sheet1!J47). |
| MLA | Zhao, Bin,et al."Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers".SMALL 9.15(2013):CONCATENATE(Sheet1!I47,-Sheet1!J47). |
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| Carbon Nanotubes Mul（1184KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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