CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers
Zhao, Bin; Yan, Juan; Wang, Dongfang; Ge, Zhilei; He, Shijiang; He, Dannong; Song, Shiping(宋世平); Fan, Chunhai(樊春海)
2013
Source PublicationSMALL
ISSN1613-6810; 1613-6829
Volume9Issue:15Pages:CONCATENATE(Sheet1!I47,-Sheet1!J47)
AbstractThere are still challenges for the development of multifunctional carbon nanotubes (CNTs). Here, a multiwalled carbon nanotube (MWCNT)-based rolling circle amplification system (CRCAS) is reported which allows in situ rolling circle replication of DNA primer on the surface of MWCNTs to create a long single-strand DNA (ssDNA) where a large number of nanoparticles or proteins could be loaded, forming a nano-biohybridized 3D structure with a powerful signal amplification ability. In this strategy, the binding ability of proteins, hybridization, replication ability of DNA, and the catalytical ability of enzymes are integrated on a single carbon nanotube. The CRCAS is then used to develop colorimetric and chemiluminescent assays for the highly sensitive and specific detection of cancer protein markers, alpha-fetoprotein (AFP) and prostate specific antigen (PSA). The colorimetric CRCAS assay is 4000 times more sensitive than a conventional enzyme-linked immunosorbent assay (ELISA), and its concentration range is 10 000 times wider. Control experiments show that as low as 10 pg mL(-1) AFP or PSA could be detected even in the presence of interfering protein markers with a more than 10(5)-fold greater concentration in the sample, demonstrating the high specificity of the CRCAS assay. The limit of detection of the chemiluminescent CRCAS assays for AFP and PSA are 5 fg mL(-1) (70 aM) and 10 fg mL(-1) (0.29 fM), respectively, indicating that the sensitivity is much higher than that of the colorimetric CRCAS assay. Importantly, CRCAS works well with real biological samples.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000327792600017
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13684
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zhao, Bin,Yan, Juan,Wang, Dongfang,et al. Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers[J]. SMALL,2013,9(15):CONCATENATE(Sheet1!I47,-Sheet1!J47).
APA Zhao, Bin.,Yan, Juan.,Wang, Dongfang.,Ge, Zhilei.,He, Shijiang.,...&Fan, Chunhai.(2013).Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers.SMALL,9(15),CONCATENATE(Sheet1!I47,-Sheet1!J47).
MLA Zhao, Bin,et al."Carbon Nanotubes Multifunctionalized by Rolling Circle Amplification and Their Application for Highly Sensitive Detection of Cancer Markers".SMALL 9.15(2013):CONCATENATE(Sheet1!I47,-Sheet1!J47).
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