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Damage of DNA ends induced by mechanical force during AFM nano-manipulation
Duan Na(段娜); Wang Xinyan; Zhang Chen; Zhang Yi(张益); Hu Jun(胡钧)
2013
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042; 2210-3147
Volume24Issue:1
AbstractAn experimental and statistical study was carried out to explore the effects of mechanical forces on the ends of linear double-stranded DNA (dsDNA) fragments. Mechanical force was applied onto individual DNA molecules during atomic force microscope (AFM)-based picking-up manipulation. By comparing the PCR efficiency of two DNA fragments with primers either at ends or at the inner regions, it was found that the ends of DNA fragments were damaged during picking-up process.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000328921400006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13700
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Duan Na,Wang Xinyan,Zhang Chen,et al. Damage of DNA ends induced by mechanical force during AFM nano-manipulation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2013,24(1).
APA Duan Na,Wang Xinyan,Zhang Chen,Zhang Yi,&Hu Jun.(2013).Damage of DNA ends induced by mechanical force during AFM nano-manipulation.NUCLEAR SCIENCE AND TECHNIQUES,24(1).
MLA Duan Na,et al."Damage of DNA ends induced by mechanical force during AFM nano-manipulation".NUCLEAR SCIENCE AND TECHNIQUES 24.1(2013).
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