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Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens
Li, Xinchun; Chen, Zuanguang; Yang, Fan; Pan, Jianbin; Li, Yinbao
2013
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume36Issue:41892Pages:CONCATENATE(Sheet1!I80,-Sheet1!J80)
AbstractL-3,4-dihydroxyphenylalanine (L-DOPA) is a well-recognized therapeutic compound to Parkinson's disease. Tyrosine is a precursor for the biosynthesis of L-DOPA, both of which are widely found in traditional medicinal material, Mucuna pruriens. In this paper, we described a validated novel analytical method based on microchip capillary electrophoresis with pulsed electrochemical detection for the simultaneous measurement of L-DOPA and tyrosine in M. pruriens. This protocol adopted end-channel amperometric detection using platinum disk electrode on a homemade glass/polydimethylsiloxane electrophoresis microchip. The background buffer consisted of 10 mM borate (pH 9.5) and 0.02 mM cetyltrimethylammonium bromide, which can produce an effective resolution for the two analytes. In the optimal condition, sufficient electrophoretic separation and sensitive detection for the target analytes can be realized within 60 s. Both tyrosine and L-DOPA yielded linear response in the concentration range of 5.0-400 M (R2 > 0.99), and the LOD were 0.79 and 1.1 M, respectively. The accuracy and precision of the established method were favorable. The present method shows several merits such as facile apparatus, high speed, low cost and minimal pollution, and provides a means for the pharmacologically active ingredients assay in M. pruriens.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000319866800013
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13710
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, Xinchun,Chen, Zuanguang,Yang, Fan,et al. Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(41892):CONCATENATE(Sheet1!I80,-Sheet1!J80).
APA Li, Xinchun,Chen, Zuanguang,Yang, Fan,Pan, Jianbin,&Li, Yinbao.(2013).Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens.JOURNAL OF SEPARATION SCIENCE,36(41892),CONCATENATE(Sheet1!I80,-Sheet1!J80).
MLA Li, Xinchun,et al."Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens".JOURNAL OF SEPARATION SCIENCE 36.41892(2013):CONCATENATE(Sheet1!I80,-Sheet1!J80).
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