CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays
Deng, Wangping; Xu, Bin; Hu, Haiyan; Li, Jianyong; Hu, Wei; Song, Shiping(宋世平); Feng, Zheng; Fan, Chunhai(樊春海)
2013
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume3
AbstractSchistosomiasis control remains to be an important and challenging task in the world. However, lack of quick, simple, sensitive and specific sero-diagnostic test is still a hurdle in the control practice. The commonly employed enzyme-linked immuno-sorbent assay (ELISA) relies on the native soluble egg antigen (SEA) that is limited in supply. Here we developed an electrochemical immunosensor array (ECISA) assay with an interfacial co-assembly strategy. A recombinant Schistosoma japonicum (Sj) calcium-binding protein (SjE16) was used as a principal antigen, while the SEA as a minor, co-assembling agent, with a ratio of 8 : 1 (SjE16: SEA, Sj16EA), which was co-immobilized on a disposable 16-channel screen-printed carbon electrode array. A portable electrochemical detector was employed to detect antibodies in serum samples. The sensitivity of ECISA reached 100% with minimal cross-reactions. Therefore, we have demonstrated that this rapid, sensitive and specific ECISA technique has the potential to perform large-scale on-site screening of Sj infection.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000318471000003
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13713
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Deng, Wangping,Xu, Bin,Hu, Haiyan,et al. Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays[J]. SCIENTIFIC REPORTS,2013,3.
APA Deng, Wangping.,Xu, Bin.,Hu, Haiyan.,Li, Jianyong.,Hu, Wei.,...&Fan, Chunhai.(2013).Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays.SCIENTIFIC REPORTS,3.
MLA Deng, Wangping,et al."Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays".SCIENTIFIC REPORTS 3(2013).
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